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Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020.
Carroll, Anne; McNamara, Eleanor.
  • Carroll A; Public Health Laboratory-Health Service Executive-Dublin, Ireland.
  • McNamara E; Public Health Laboratory-Health Service Executive-Dublin, Ireland.
Euro Surveill ; 26(6)2021 02.
Article in English | MEDLINE | ID: covidwho-1376681
ABSTRACT
We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if Ct values across platforms are comparable. We also investigate whether a Ct value, a surrogate for calculated viral loads in the absence of viral culture of > 34 can be used to exclude SARS-CoV-2 infection and its complications. We found a variation in Ct values from different assays for the same calculated viral load; this should be taken into consideration for result interpretation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Reagent Kits, Diagnostic / Real-Time Polymerase Chain Reaction / SARS-CoV-2 Type of study: Diagnostic study Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: 1560-7917.ES.2021.26.6.2002079

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Reagent Kits, Diagnostic / Real-Time Polymerase Chain Reaction / SARS-CoV-2 Type of study: Diagnostic study Limits: Humans Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: 1560-7917.ES.2021.26.6.2002079