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Heme oxygenase-1 inducer hemin does not inhibit SARS-CoV-2 virus infection.
Maestro, Sheila; Córdoba, Karol M; Olague, Cristina; Argemi, Josepmaria; Ávila, Matías A; González-Aseguinolaza, Gloria; Smerdou, Cristian; Fontanellas, Antonio.
  • Maestro S; Gene Therapy and Regulation of Gene Expression Program, CIMA Universidad de Navarra, Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.
  • Córdoba KM; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain; Hepatology Program, CIMA Universidad de Navarra, Pamplona, Spain.
  • Olague C; Gene Therapy and Regulation of Gene Expression Program, CIMA Universidad de Navarra, Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.
  • Argemi J; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain; Hepatology Program, CIMA Universidad de Navarra, Pamplona, Spain; National Institute for the Study of Liver and Gastrointestinal Diseases (CIBERehd, Carlos III Health Institute), Madrid, Spain.
  • Ávila MA; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain; Hepatology Program, CIMA Universidad de Navarra, Pamplona, Spain; National Institute for the Study of Liver and Gastrointestinal Diseases (CIBERehd, Carlos III Health Institute), Madrid, Spain.
  • González-Aseguinolaza G; Gene Therapy and Regulation of Gene Expression Program, CIMA Universidad de Navarra, Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.
  • Smerdou C; Gene Therapy and Regulation of Gene Expression Program, CIMA Universidad de Navarra, Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain. Electronic address: csmerdou@unav.es.
  • Fontanellas A; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain; Hepatology Program, CIMA Universidad de Navarra, Pamplona, Spain; National Institute for the Study of Liver and Gastrointestinal Diseases (CIBERehd, Carlos III Health Institute), Madrid, Spain. Electronic address: afontanellas@unav.es.
Biomed Pharmacother ; 137: 111384, 2021 May.
Article in English | MEDLINE | ID: covidwho-1082719
ABSTRACT
Antiviral agents with different mechanisms of action could induce synergistic effects against SARS-CoV-2 infection. Some reports suggest the therapeutic potential of the heme oxygenase-1 (HO-1) enzyme against virus infection. Given that hemin is a natural inducer of the HO-1 gene, the aim of this study was to develop an in vitro assay to analyze the antiviral potency of hemin against SARS-CoV-2 infection. A SARS-CoV-2 infectivity assay was conducted in Vero-E6 and Calu-3 epithelial cell lines. The antiviral effect of hemin, and chloroquine as a control, against SARS-CoV-2 virus infection was quantified by RT-qPCR using specific oligonucleotides for the N gene. Chloroquine induced a marked reduction of viral genome copies in kidney epithelial Vero-E6 cells but not in lung cancer Calu-3 cells. Hemin administration to the culture medium induced a high induction in the expression of the HO-1 gene that was stronger in Vero-E6 macaque-derived cells than in the human Calu-3 cell line. However, hemin treatment did not modify SARS-CoV-2 replication, as measured by viral genome quantification 48 h post-infection for Vero-E6 and 72 h post-infection for the Calu-3 lineages. In conclusion, although exposure to hemin induced strong HO-1 up-regulation, this effect was unable to inhibit or delay the progression of SARS-CoV-2 infection in two epithelial cell lines susceptible to infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Heme Oxygenase-1 / SARS-CoV-2 / Hemin Limits: Animals / Humans Language: English Journal: Biomed Pharmacother Year: 2021 Document Type: Article Affiliation country: J.biopha.2021.111384

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Heme Oxygenase-1 / SARS-CoV-2 / Hemin Limits: Animals / Humans Language: English Journal: Biomed Pharmacother Year: 2021 Document Type: Article Affiliation country: J.biopha.2021.111384