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Administration of high titer convalescent anti-SARS-CoV-2 plasma: From donor selection to monitoring recipient outcomes.
Mendoza, Rachelle P; Fyke, William; Daniel, David; Gabutan, Elmer; Das, Ballabh; Bajaj, Harsha; Easy, Maxine; Vasileva, Ana; Colbourn, Robert; Alawad, Mouyed; Dehghani, Amir; Lin, Bo; Emechebe, Dokpe; Patel, Purvi; Jabbar, Absia; Nikolov, Dimitar B; Giovaniello, Dominick; Kang, Steven; Tatem, Luis; Bromberg, Kenneth; Augenbraun, Michael; Premsrirut, Prem; Libien, Jenny; Norin, Allen J.
  • Mendoza RP; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Fyke W; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Graduate Program in Neural and Behavioral Science, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Daniel D; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Gabutan E; Mirimus Inc., 760 Parkside Ave., Brooklyn, NY 11226, USA.
  • Das B; Department of Medicine, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Bajaj H; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Easy M; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Vasileva A; Mirimus Inc., 760 Parkside Ave., Brooklyn, NY 11226, USA; Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Colbourn R; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Graduate Program in Neural and Behavioral Science, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Alawad M; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Dehghani A; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Lin B; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Emechebe D; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Patel P; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Jabbar A; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Nikolov DB; Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Ave., New York, NY 10065, USA.
  • Giovaniello D; Brooklyn Blood Donation Center, Maimonides Medical Center, 4802 10th Ave, Brooklyn, NY 11219, USA.
  • Kang S; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Tatem L; Department of Medicine, Division of Infectious Disease, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Bromberg K; Department of Pediatric Infectious Diseases, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Augenbraun M; Department of Medicine, Division of Infectious Disease, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Premsrirut P; Mirimus Inc., 760 Parkside Ave., Brooklyn, NY 11226, USA; Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Libien J; Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA.
  • Norin AJ; Department of Medicine, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA; Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11203, USA. Electronic address: allen.norin@downstate.edu.
Hum Immunol ; 82(4): 255-263, 2021 Apr.
Article in English | MEDLINE | ID: covidwho-1085551
ABSTRACT
Early in the SARS-CoV-2 pandemic, convalescent plasma (CP) therapy was proposed as a treatment for severely ill patients. We conducted a CP treatment protocol under the Mayo Clinic Extended Access Program at University Hospital Brooklyn (UHB). Potential donors were screened with a lateral flow assay (LFA) for IgM and IgG antibodies against the SARS-CoV-2 S1 receptor-binding domain (RBD). Volunteers that were LFA positive were tested with an ELISA to measure IgG titers against the RBD. Subjects with titers of at least 11024 were selected to donate. Most donors with positive LFA had acceptable titers and were eligible to donate. Out of 171 volunteers, only 65 tested positive in the LFA (38.0%), and 55 (32.2%) had titers of at least 11024. Before our donation program started, 31 CP units were procured from the New York Blood Center (NYBC). Among the 31 CP units that were obtained from the NYBC, 25 units (80.6%) were positive in the LFA but only 12 units (38.7%) had titers of at least 11024. CP was administered to 28 hospitalized COVID-19 patients. Patients who received low titer CP, high titer CP and patients who did not receive CP were followed for 45 days after presentation. Severe adverse events were not associated with CP transfusion. Death was a less frequent outcome for patients that received high titer CP (>11024) 38.6% mortality, than patients that received low titer CP (≤11024) 77.8% mortality.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Hum Immunol Year: 2021 Document Type: Article Affiliation country: J.humimm.2021.02.007

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Hum Immunol Year: 2021 Document Type: Article Affiliation country: J.humimm.2021.02.007