Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro.
Biol Cell
; 113(6): 281-293, 2021 Jun.
Article
in English
| MEDLINE | ID: covidwho-1087985
ABSTRACT
BACKGROUND INFORMATION Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from São Paulo state (Brazil). RESULTS:
Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space. CONCLUSIONS ANDSIGNIFICANCE:
This study contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles and cell components, in a context of a virus-induced membrane remodelling.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Endoplasmic Reticulum
/
SARS-CoV-2
/
Intracellular Membranes
/
Nuclear Envelope
Limits:
Animals
/
Humans
Language:
English
Journal:
Biol Cell
Year:
2021
Document Type:
Article
Affiliation country:
Boc.202000146
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