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The Architecture of SARS-CoV-2 Transcriptome.
Kim, Dongwan; Lee, Joo-Yeon; Yang, Jeong-Sun; Kim, Jun Won; Kim, V Narry; Chang, Hyeshik.
  • Kim D; Center for RNA Research, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea.
  • Lee JY; Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 28159, Republic of Korea.
  • Yang JS; Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 28159, Republic of Korea.
  • Kim JW; Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Osong 28159, Republic of Korea.
  • Kim VN; Center for RNA Research, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: narrykim@snu.ac.kr.
  • Chang H; Center for RNA Research, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: hyeshik@snu.ac.kr.
Cell ; 181(4): 914-921.e10, 2020 05 14.
Article in English | MEDLINE | ID: covidwho-108856
ABSTRACT
SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Transcriptome / Betacoronavirus Limits: Animals Language: English Journal: Cell Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Transcriptome / Betacoronavirus Limits: Animals Language: English Journal: Cell Year: 2020 Document Type: Article