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Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy.
Emerg Infect Dis ; 27(4): 1023-1031, 2021 04.
Article in English | MEDLINE | ID: covidwho-1088897
ABSTRACT
Efforts to combat the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have placed a renewed focus on the use of transmission electron microscopy for identifying coronavirus in tissues. In attempts to attribute pathology of COVID-19 patients directly to tissue damage caused by SARS-CoV-2, investigators have inaccurately reported subcellular structures, including coated vesicles, multivesicular bodies, and vesiculating rough endoplasmic reticulum, as coronavirus particles. We describe morphologic features of coronavirus that distinguish it from subcellular structures, including particle size range (60-140 nm), intracellular particle location within membrane-bound vacuoles, and a nucleocapsid appearing in cross section as dense dots (6-12 nm) within the particles. In addition, although the characteristic spikes of coronaviruses may be visible on the virus surface, especially on extracellular particles, they are less evident in thin sections than in negative stain preparations.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cellular Structures / SARS-CoV-2 / COVID-19 Type of study: Randomized controlled trials Limits: Humans Language: English Journal: Emerg Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Cellular Structures / SARS-CoV-2 / COVID-19 Type of study: Randomized controlled trials Limits: Humans Language: English Journal: Emerg Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article