Your browser doesn't support javascript.
A low level of CD16pos monocytes in SARS-CoV-2 infected patients is a marker of severity.
Vasse, Marc; Zuber, Benjamin; Goubeau, Laurie; Ballester, Marie-Christine; Roumier, Mathilde; Delcominette, Frédérique; Habarou, Florence; Jolly, Emilie; Ackermann, Felix; Cerf, Charles; Farfour, Eric; Pascreau, Tiffany.
  • Vasse M; Biology Department, Foch Hospital, Suresnes, France.
  • Zuber B; UMRS-1176, Le Kremlin-Bicêtre, France.
  • Goubeau L; Intensive Care Unit, Foch Hospital, Suresnes, France.
  • Ballester MC; Biology Department, Foch Hospital, Suresnes, France.
  • Roumier M; Emergency Unit, Foch Hospital, Suresnes, France.
  • Delcominette F; Internal Medicine Department, Foch Hospital, Suresnes, France.
  • Habarou F; Biology Department, Foch Hospital, Suresnes, France.
  • Jolly E; Biology Department, Foch Hospital, Suresnes, France.
  • Ackermann F; Biology Department, Foch Hospital, Suresnes, France.
  • Cerf C; Internal Medicine Department, Foch Hospital, Suresnes, France.
  • Farfour E; Intensive Care Unit, Foch Hospital, Suresnes, France.
  • Pascreau T; Biology Department, Foch Hospital, Suresnes, France.
Clin Chem Lab Med ; 59(7): 1315-1322, 2021 Jun 25.
Article in English | MEDLINE | ID: covidwho-1278216
ABSTRACT

OBJECTIVES:

Severe forms of coronavirus disease 2019 (COVID-19) are characterized by an excessive production of inflammatory cytokines. Activated monocytes secrete high levels of cytokines. Human monocytes are divided into three major populations conventional (CD14posCD16neg), non-classical (CD14dimCD16pos), and intermediate (CD14posCD16pos) monocytes. The aim of this study was to analyze whether the distribution of conventional (CD16neg) and CD16pos monocytes is different in patients with COVID-19 and whether the variations could be predictive of the outcome of the disease.

METHODS:

We performed a prospective study on 390 consecutive patients referred to the Emergency Unit, with a proven diagnosis of SARS-CoV 2 infection by RT-PCR. Using the CytoDiff™ reagent, an automated routine leukocyte differential, we quantified CD16neg and CD16pos monocytes.

RESULTS:

In the entire population, median CD16neg and CD16pos monocyte levels (0.398 and 0.054×109/L, respectively) were in the normal range [(0.3-0.7×109/L) and (0.015-0.065×109/L), respectively], but the 35 patients in the intensive care unit (ICU) had a significantly (p<0.001) lower CD16pos monocyte count (0.018 × 109/L) in comparison to the 70 patients who were discharged (0.064 × 109/L) or were hospitalized in conventional units (0.058 × 109/L). By ROC curve analysis, the ratio [absolute neutrophil count/CD16pos monocyte count] was highly discriminant to identify patients requiring ICU hospitalization with a cut-off 193.1, the sensitivity and the specificity were 74.3 and 81.8%, respectively (area under the curve=0.817).

CONCLUSIONS:

Quantification of CD16pos monocytes and the ratio [absolute neutrophil count/CD16pos monocyte count] could constitute a marker of the severity of disease in COVID-19 patients.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Monocytes / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2021 Document Type: Article Affiliation country: Cclm-2020-1801

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Monocytes / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Chem Lab Med Journal subject: Chemistry, Clinical / Laboratory Techniques and procedures Year: 2021 Document Type: Article Affiliation country: Cclm-2020-1801