Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA.
Clin Lab
; 67(2)2021 Feb 01.
Article
in English
| MEDLINE | ID: covidwho-1094345
ABSTRACT
BACKGROUND AND METHODS:
2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality.RESULTS:
In this study, we found that unmatched virus preservation solution will inhibit N gene and OFR-1ab gene (two independent genes of SARS-CoV-2) amplification in one-step detection reagent.CONCLUSIONS:
Despite just being a particular phenomenon we found in our work to fight 2019-nCoV, we concluded that unmatched virus preservation solution may have an inhibitory effect on SARS-CoV-2 nucleic acid detection which may lead to incorrect clinical diagnosis.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Specimen Handling
/
Organ Preservation Solutions
/
Genes, Viral
/
COVID-19 Nucleic Acid Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal subject:
Laboratory Techniques and procedures
Year:
2021
Document Type:
Article
Similar
MEDLINE
...
LILACS
LIS