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Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus.
Pramanik, Avijit; Gao, Ye; Patibandla, Shamily; Mitra, Dipanwita; McCandless, Martin G; Fassero, Lauren A; Gates, Kalein; Tandon, Ritesh; Ray, Paresh Chandra.
  • Pramanik A; Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
  • Gao Y; Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
  • Patibandla S; Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
  • Mitra D; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi 39216, United States.
  • McCandless MG; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi 39216, United States.
  • Fassero LA; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi 39216, United States.
  • Gates K; Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
  • Tandon R; Department of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, Mississippi 39216, United States.
  • Ray PC; Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi 39217, United States.
J Phys Chem Lett ; 12(8): 2166-2171, 2021 Mar 04.
Article in English | MEDLINE | ID: covidwho-1101616
ABSTRACT
The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer-spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Aptamers, Nucleotide / Metal Nanoparticles / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Gold / Antigens, Viral Type of study: Diagnostic study Topics: Long Covid Limits: Humans Language: English Journal: J Phys Chem Lett Year: 2021 Document Type: Article Affiliation country: Acs.jpclett.0c03570

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Aptamers, Nucleotide / Metal Nanoparticles / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Gold / Antigens, Viral Type of study: Diagnostic study Topics: Long Covid Limits: Humans Language: English Journal: J Phys Chem Lett Year: 2021 Document Type: Article Affiliation country: Acs.jpclett.0c03570