Ginkgolic acid and anacardic acid are specific covalent inhibitors of SARS-CoV-2 cysteine proteases.
Cell Biosci
; 11(1): 45, 2021 Feb 28.
Article
in English
| MEDLINE | ID: covidwho-1105737
ABSTRACT
BACKGROUND:
In the urgent campaign to develop therapeutics against SARS-CoV-2, natural products have been an important source of new lead compounds.RESULTS:
We herein identified two natural products, ginkgolic acid and anacardic acid, as inhibitors using a high-throughput screen targeting the SARS-CoV-2 papain-like protease (PLpro). Moreover, our study demonstrated that the two hit compounds are dual inhibitors targeting the SARS-CoV-2 3-chymotrypsin-like protease (3CLpro) in addition to PLpro. A mechanism of action study using enzyme kinetics further characterized the two compounds as irreversible inhibitors against both 3CLpro and PLpro. Significantly, both identified compounds inhibit SARS-CoV-2 replication in vitro at nontoxic concentrations.CONCLUSIONS:
Our finding provides two novel natural products as promising SARS-CoV-2 antivirals.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
Cell Biosci
Year:
2021
Document Type:
Article
Affiliation country:
S13578-021-00564-x
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