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An Optimized High-Throughput Immuno-Plaque Assay for SARS-CoV-2.
Amarilla, Alberto A; Modhiran, Naphak; Setoh, Yin Xiang; Peng, Nias Y G; Sng, Julian D J; Liang, Benjamin; McMillan, Christopher L D; Freney, Morgan E; Cheung, Stacey T M; Chappell, Keith J; Khromykh, Alexander A; Young, Paul R; Watterson, Daniel.
  • Amarilla AA; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Modhiran N; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Setoh YX; The Australian Institute for Biotechnology and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia.
  • Peng NYG; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Sng JDJ; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Liang B; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • McMillan CLD; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Freney ME; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Cheung STM; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Chappell KJ; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Khromykh AA; School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
  • Young PR; The Australian Institute for Biotechnology and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia.
  • Watterson D; Australian Infectious Disease Research Centre, The University of Queensland, St Lucia, QLD, Australia.
Front Microbiol ; 12: 625136, 2021.
Article in English | MEDLINE | ID: covidwho-1110305
Semantic information from SemMedBD (by NLM)
1. Detection USES Antibodies
Subject
Detection
Predicate
USES
Object
Antibodies
2. Detection USES Antibodies
Subject
Detection
Predicate
USES
Object
Antibodies
ABSTRACT
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable of human-to-human transmission and rapid global spread. The rapid emergence and global spread of SARS-CoV-2 has encouraged the establishment of a rapid, sensitive, and reliable viral detection and quantification methodology. Here, we present an alternative assay, termed immuno-plaque assay (iPA), which utilizes a combination of plaque assay and immunofluorescence techniques. We have extensively optimized the conditions for SARS-CoV-2 infection and demonstrated the great flexibility of iPA detection using several antibodies and dual-probing with two distinct epitope-specific antibodies. In addition, we showed that iPA could be utilized for ultra-high-throughput viral titration and neutralization assay within 24 h and is amenable to a 384-well format. These advantages will significantly accelerate SARS-CoV-2 research outcomes during this pandemic period.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Front Microbiol Year: 2021 Document Type: Article Affiliation country: Fmicb.2021.625136

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Front Microbiol Year: 2021 Document Type: Article Affiliation country: Fmicb.2021.625136