Real-time PCR assay as a simple and efficient tool for viral stability study.
Bioanalysis
; 13(5): 387-394, 2021 Mar.
Article
in English
| MEDLINE | ID: covidwho-1116300
ABSTRACT
Aim:
For oncolytic virus trials, regulatory agencies often require pharmaceutical industry to evaluate risks of released viruses from patients to environment. This study was to establish a real-time PCR method to assess viral shedding and viral stability in human urine. Results/methodology:
Herein, we describe an incubation of viral drug product in human urine and use of real-time PCR as a simple, efficient and high throughput assay to assess the level and stability of a nonenveloped and single stranded RNA virus. The viral stability issue is critical to the collection, transport, storage and testing of clinical samples. Discussion/conclusion:
In summary, this simple method provides useful viral stability information at various temperatures and detergents. A similar approach may apply to other RNA viruses (including SARS-CoV-2).Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Virus Diseases
/
RNA, Viral
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Bioanalysis
Year:
2021
Document Type:
Article
Affiliation country:
Bio-2021-0002
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