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Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva.
Mencucci, Rita; Favuzza, Eleonora; Becatti, Matteo; Tani, Alessia; Mazzantini, Costanza; Vignapiano, Roberto; Fiorillo, Claudia; Pellegrini-Giampietro, DomenicoE; Manetti, Mirko; Marini, Mirca; Landucci, Elisa.
  • Mencucci R; Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: rita.mencucci@unifi.it.
  • Favuzza E; Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: elefavuzza@gmail.com.
  • Becatti M; Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Viale Morgagni 50, 50134, Florence, Italy. Electronic address: matteo.becatti@unifi.it.
  • Tani A; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: alessia.tani@unifi.it.
  • Mazzantini C; Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: costanza.mazzantini@unifi.it.
  • Vignapiano R; Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: roberto.vignapiano@gmail.com.
  • Fiorillo C; Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Viale Morgagni 50, 50134, Florence, Italy. Electronic address: claudia.fiorillo@unifi.it.
  • Pellegrini-Giampietro D; Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: domenico.pellegrini@unifi.it.
  • Manetti M; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: mirko.manetti@unifi.it.
  • Marini M; Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: mirca.marini@unifi.it.
  • Landucci E; Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: elisa.landucci@unifi.it.
Exp Eye Res ; 205: 108527, 2021 04.
Article in English | MEDLINE | ID: covidwho-1116639
ABSTRACT
The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were expressed in human immortalized conjunctival cells, and this was confirmed by confocal microscopy images, that demonstrated a marked cellular expression of the viral receptors and their co-localization on the cell membranes. Healthy conjunctival samples from 11 adult patients were excised during retinal detachment surgery. We found the expression of ACE2 and TMPRSS2 in all the conjunctival surgical specimens analyzed and their co-localization in the superficial conjunctival epithelium. The ACE2 Western blot levels and immunofluorescence staining for ACE2 were variable among specimens. These results suggest the susceptibility of the conjunctival epithelium to SARS-CoV-2 infection, even though with a possible interindividual variability.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Serine Endopeptidases / Gene Expression Regulation / Peptidyl-Dipeptidase A / Conjunctiva / Epithelial Cells / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Exp Eye Res Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Serine Endopeptidases / Gene Expression Regulation / Peptidyl-Dipeptidase A / Conjunctiva / Epithelial Cells / COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Exp Eye Res Year: 2021 Document Type: Article