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The Potential of Developing Pan-Coronaviral Antibodies to Spike Peptides in Convalescent COVID-19 Patients.
Rabets, Andrii; Bila, Galyna; Grytsko, Roman; Samborskyy, Markian; Rebets, Yuriy; Vari, Sandor G; Pagneux, Quentin; Barras, Alexandre; Boukherroub, Rabah; Szunerits, Sabine; Bilyy, Rostyslav.
  • Rabets A; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
  • Bila G; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
  • Grytsko R; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
  • Samborskyy M; Explogen LLC, Lviv, Ukraine.
  • Rebets Y; Explogen LLC, Lviv, Ukraine.
  • Vari SG; International Research and Innovation in Medicine Program, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
  • Pagneux Q; University of Lille, CNRS, Centrale Lille, University of Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, France.
  • Barras A; University of Lille, CNRS, Centrale Lille, University of Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, France.
  • Boukherroub R; University of Lille, CNRS, Centrale Lille, University of Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, France.
  • Szunerits S; University of Lille, CNRS, Centrale Lille, University of Polytechnique Hauts-de-France, UMR 8520, IEMN, Lille, France.
  • Bilyy R; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine. r.bilyy@gmail.com.
Arch Immunol Ther Exp (Warsz) ; 69(1): 5, 2021 Mar 06.
Article in English | MEDLINE | ID: covidwho-1118194
Semantic information from SemMedBD (by NLM)
1. Peptides TREATS COVID-19
Subject
Peptides
Predicate
TREATS
Object
COVID-19
2. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
3. Peptides TREATS Patients
Subject
Peptides
Predicate
TREATS
Object
Patients
4. Protein Subunits INTERACTS_WITH Cells
Subject
Protein Subunits
Predicate
INTERACTS_WITH
Object
Cells
5. Protein Subunits AFFECTS Membrane Fusion
Subject
Protein Subunits
Predicate
AFFECTS
Object
Membrane Fusion
6. Middle East Respiratory Syndrome Coronavirus LOCATION_OF Peptides
Subject
Middle East Respiratory Syndrome Coronavirus
Predicate
LOCATION_OF
Object
Peptides
7. M Protei PART_OF C3698360
Subject
M Protei
Predicate
PART_OF
Object
C3698360
8. Peptides PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
Peptides
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
9. Specimen Type - Serum PART_OF Patients
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Patients
10. immunoglobulin G TREATS COVID-19
Subject
immunoglobulin G
Predicate
TREATS
Object
COVID-19
11. immunoglobulin G TREATS Patients
Subject
immunoglobulin G
Predicate
TREATS
Object
Patients
12. Blood specimen LOCATION_OF Antibodies
Subject
Blood specimen
Predicate
LOCATION_OF
Object
Antibodies
13. Peptides COEXISTS_WITH Antibodies
Subject
Peptides
Predicate
COEXISTS_WITH
Object
Antibodies
14. Polyclonal antibody PREVENTS Middle East Respiratory Syndrome Coronavirus
Subject
Polyclonal antibody
Predicate
PREVENTS
Object
Middle East Respiratory Syndrome Coronavirus
15. Coronavirus Infections PRODUCES Antibodies
Subject
Coronavirus Infections
Predicate
PRODUCES
Object
Antibodies
16. Peptides TREATS COVID-19
Subject
Peptides
Predicate
TREATS
Object
COVID-19
17. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
18. Peptides TREATS Patients
Subject
Peptides
Predicate
TREATS
Object
Patients
19. Protein Subunits INTERACTS_WITH Cells
Subject
Protein Subunits
Predicate
INTERACTS_WITH
Object
Cells
20. Protein Subunits AFFECTS Membrane Fusion
Subject
Protein Subunits
Predicate
AFFECTS
Object
Membrane Fusion
21. Middle East Respiratory Syndrome Coronavirus LOCATION_OF Peptides
Subject
Middle East Respiratory Syndrome Coronavirus
Predicate
LOCATION_OF
Object
Peptides
22. M Protein, multiple myeloma PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
M Protein, multiple myeloma
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
23. Peptides PART_OF Middle East Respiratory Syndrome Coronavirus
Subject
Peptides
Predicate
PART_OF
Object
Middle East Respiratory Syndrome Coronavirus
24. Specimen Type - Serum PART_OF Patients
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Patients
25. immunoglobulin G TREATS COVID-19
Subject
immunoglobulin G
Predicate
TREATS
Object
COVID-19
26. immunoglobulin G TREATS Patients
Subject
immunoglobulin G
Predicate
TREATS
Object
Patients
27. Blood specimen LOCATION_OF Antibodies
Subject
Blood specimen
Predicate
LOCATION_OF
Object
Antibodies
28. Peptides COEXISTS_WITH Antibodies
Subject
Peptides
Predicate
COEXISTS_WITH
Object
Antibodies
29. Polyclonal antibody PREVENTS Middle East Respiratory Syndrome Coronavirus
Subject
Polyclonal antibody
Predicate
PREVENTS
Object
Middle East Respiratory Syndrome Coronavirus
30. Coronavirus Infections PRODUCES Antibodies
Subject
Coronavirus Infections
Predicate
PRODUCES
Object
Antibodies
ABSTRACT
Coronaviruses share conservative spike protein (S) on their enveloped membrane surface, where S1 subunit recognizes and binds the cellular receptor, and the S2 subunit mediates membrane fusion. This similarity raises the question does coronaviral infection by one create protection to others? Convalescent SARS-CoV-2 (COVID-19) sera were tested for cross reactivity with peptides from Middle East respiratory syndrome coronavirus (MERS-CoV) which shares 74% homology. Our results showed significant cross-reactivity with a peptide of the heptad repeat 2 (HR2) domain of the MERS-CoV spike protein. Sera samples of 47 validated seropositive convalescent COVID-19 patients and 40 sera samples of control patients, collected in pre-COVID time were used to establish cross-bind reactivity with the MERS-CoV peptide. Significantly stronger binding (p < 0.0001) was observed for IgG antibodies in convalescent COVID-19 patients compared to the control group. In ELISA, MERS-CoV peptide helps to discriminate post-COVID-19 populations and non-infected ones by the presence of antibodies in blood samples. This suggests that polyclonal antibodies established during SARS-CoV-2 infection can recognize and probably decrease severity of MERS-CoV and other coronaviral infections. The high homology of the spike protein domain also suggests that the opposite effect can be true coronaviral infections produce cross-reactive antibodies effective against SARS-CoV-2. The collected data prove that despite the core HR2 region is hidden in the native viral conformation, its exposure during cell entry makes it highly immunogenic. Since inhibitory peptides to this region were previously described, this opens new possibilities in fighting coronaviral infections and developing vaccines effective even after possible viral mutations.
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Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Convalescence / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Randomized controlled trials Topics: Long Covid / Vaccines Limits: Humans Language: English Journal: Arch Immunol Ther Exp (Warsz) Year: 2021 Document Type: Article Affiliation country: S00005-021-00607-8

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Convalescence / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Observational study / Randomized controlled trials Topics: Long Covid / Vaccines Limits: Humans Language: English Journal: Arch Immunol Ther Exp (Warsz) Year: 2021 Document Type: Article Affiliation country: S00005-021-00607-8