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Ubiquitin-Modified Proteome of SARS-CoV-2-Infected Host Cells Reveals Insights into Virus-Host Interaction and Pathogenesis.
Zhang, Huan; Zheng, Huanying; Zhu, Jinying; Dong, Qiao; Wang, Jin; Fan, Huahao; Chen, Yangzhen; Zhang, Xi; Han, Xiaohu; Li, Qianlin; Lu, Jiahai; Tong, Yigang; Chen, Zeliang.
  • Zhang H; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
  • Zheng H; Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, P. R. China.
  • Zhu J; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
  • Dong Q; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
  • Wang J; School of Public Health, Sun Yat-sen University, Guangzhou 510080, P. R. China.
  • Fan H; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.
  • Chen Y; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.
  • Zhang X; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
  • Han X; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
  • Li Q; School of Public Health, Sun Yat-sen University, Guangzhou 510080, P. R. China.
  • Lu J; School of Public Health, Sun Yat-sen University, Guangzhou 510080, P. R. China.
  • Tong Y; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China.
  • Chen Z; Key Laboratory of Zoonotic of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, P. R. China.
J Proteome Res ; 20(5): 2224-2239, 2021 05 07.
Article in English | MEDLINE | ID: covidwho-1118785
ABSTRACT
The outbreak of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health. The mechanism of pathogenesis and the host immune response to SARS-CoV-2 infection are largely unknown. In the present study, we applied a quantitative proteomic technology to identify and quantify the ubiquitination changes that occur in both the virus and the Vero E6 cells during SARS-CoV-2 infection. By applying label-free, quantitative liquid chromatography with tandem mass spectrometry proteomics, 8943 lysine ubiquitination sites on 3086 proteins were identified, of which 138 sites on 104 proteins were quantified as significantly upregulated, while 828 sites on 447 proteins were downregulated at 72 h post-infection. Bioinformatics analysis suggested that SARS-CoV-2 infection might modulate host immune responses through the ubiquitination of important proteins, including USP5, IQGAP1, TRIM28, and Hsp90. Ubiquitination modification was also observed on 11 SAR-CoV-2 proteins, including proteins involved in virus replication and inhibition of the host innate immune response. Our study provides new insights into the interaction between SARS-CoV-2 and the host as well as potential targets for the prevention and treatment of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Proteome / COVID-19 Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Proteome / COVID-19 Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2021 Document Type: Article