Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.
Nat Commun
; 12(1): 1467, 2021 03 05.
Article
in English
| MEDLINE | ID: covidwho-1118805
ABSTRACT
Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Colorimetry
/
Nucleic Acid Amplification Techniques
/
Molecular Diagnostic Techniques
/
Point-of-Care Testing
/
COVID-19 Testing
/
SARS-CoV-2
/
COVID-19
/
Nucleic Acid Hybridization
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Nat Commun
Journal subject:
Biology
/
Science
Year:
2021
Document Type:
Article
Affiliation country:
S41467-021-21627-0
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