Your browser doesn't support javascript.
Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies.
Chen, Rita E; Zhang, Xianwen; Case, James Brett; Winkler, Emma S; Liu, Yang; VanBlargan, Laura A; Liu, Jianying; Errico, John M; Xie, Xuping; Suryadevara, Naveenchandra; Gilchuk, Pavlo; Zost, Seth J; Tahan, Stephen; Droit, Lindsay; Turner, Jackson S; Kim, Wooseob; Schmitz, Aaron J; Thapa, Mahima; Wang, David; Boon, Adrianus C M; Presti, Rachel M; O'Halloran, Jane A; Kim, Alfred H J; Deepak, Parakkal; Pinto, Dora; Fremont, Daved H; Crowe, James E; Corti, Davide; Virgin, Herbert W; Ellebedy, Ali H; Shi, Pei-Yong; Diamond, Michael S.
  • Chen RE; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Zhang X; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Case JB; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
  • Winkler ES; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Liu Y; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • VanBlargan LA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Liu J; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
  • Errico JM; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Xie X; Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
  • Suryadevara N; Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, USA.
  • Gilchuk P; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Zost SJ; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
  • Tahan S; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Droit L; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Turner JS; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Kim W; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
  • Schmitz AJ; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
  • Thapa M; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Wang D; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Boon ACM; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Presti RM; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • O'Halloran JA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
  • Kim AHJ; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Deepak P; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Pinto D; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
  • Fremont DH; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Crowe JE; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Corti D; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Virgin HW; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
  • Ellebedy AH; Humabs BioMed SA, a subsidiary of Vir Biotechnology, Bellinzona, Switzerland.
  • Shi PY; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.
  • Diamond MS; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA.
Nat Med ; 27(4): 717-726, 2021 04.
Article in English | MEDLINE | ID: covidwho-1118812
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the global COVID-19 pandemic. Rapidly spreading SARS-CoV-2 variants may jeopardize newly introduced antibody and vaccine countermeasures. Here, using monoclonal antibodies (mAbs), animal immune sera, human convalescent sera and human sera from recipients of the BNT162b2 mRNA vaccine, we report the impact on antibody neutralization of a panel of authentic SARS-CoV-2 variants including a B.1.1.7 isolate, chimeric strains with South African or Brazilian spike genes and isogenic recombinant viral variants. Many highly neutralizing mAbs engaging the receptor-binding domain or N-terminal domain and most convalescent sera and mRNA vaccine-induced immune sera showed reduced inhibitory activity against viruses containing an E484K spike mutation. As antibodies binding to spike receptor-binding domain and N-terminal domain demonstrate diminished neutralization potency in vitro against some emerging variants, updated mAb cocktails targeting highly conserved regions, enhancement of mAb potency or adjustments to the spike sequences of vaccines may be needed to prevent loss of protection in vivo.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / SARS-CoV-2 / Antibodies, Monoclonal / Antibodies, Viral Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Nat Med Journal subject: Molecular Biology / Medicine Year: 2021 Document Type: Article Affiliation country: S41591-021-01294-w

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / SARS-CoV-2 / Antibodies, Monoclonal / Antibodies, Viral Topics: Vaccines / Variants Limits: Animals / Humans Language: English Journal: Nat Med Journal subject: Molecular Biology / Medicine Year: 2021 Document Type: Article Affiliation country: S41591-021-01294-w