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Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin.
Emmerich, Petra; Murawski, Carolin; Ehmen, Christa; von Possel, Ronald; Pekarek, Neele; Oestereich, Lisa; Duraffour, Sophie; Pahlmann, Meike; Struck, Nicole; Eibach, Daniel; Krumkamp, Ralf; Amuasi, John; Maiga-Ascofaré, Oumou; Rakotozandrindrainy, Raphael; Asogun, Danny; Ighodalo, Yemisi; Kann, Simone; May, Jürgen; Tannich, Egbert; Deschermeier, Christina.
  • Emmerich P; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Murawski C; Department of Tropical Medicine and Infectious Diseases, Center of Internal Medicine II, University of Rostock, Rostock, Germany.
  • Ehmen C; Department for Infectious Disease Diagnostics, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • von Possel R; Department for Infectious Disease Diagnostics, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Pekarek N; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Oestereich L; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Duraffour S; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Pahlmann M; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • Struck N; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Eibach D; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • Krumkamp R; Department for Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Amuasi J; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • Maiga-Ascofaré O; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • Rakotozandrindrainy R; Department for Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Asogun D; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • Ighodalo Y; Department for Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Kann S; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
  • May J; Department for Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
  • Tannich E; Global Health and Infectious Disease Research Group, Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Deschermeier C; German Center for Infection Research, Hamburg - Lübeck - Borstel - Riems, Germany.
Trop Med Int Health ; 26(6): 621-631, 2021 06.
Article in English | MEDLINE | ID: covidwho-1119265
Preprint
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Semantic information from SemMedBD (by NLM)
1. Specimen Type - Serum PART_OF Plasma
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Plasma
2. Enzyme-Linked Immunosorbent Assay USES Nucleocapsid
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
Nucleocapsid
3. Enzyme-Linked Immunosorbent Assay USES SARS-CoV-2 IgG
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
SARS-CoV-2 IgG
4. Serum LOCATION_OF immunoglobulin G
Subject
Serum
Predicate
LOCATION_OF
Object
immunoglobulin G
5. Antigens DISRUPTS Immune response
Subject
Antigens
Predicate
DISRUPTS
Object
Immune response
6. Specimen Type - Serum PART_OF Plasma
Subject
Specimen Type - Serum
Predicate
PART_OF
Object
Plasma
7. Enzyme-Linked Immunosorbent Assay USES Nucleocapsid
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
Nucleocapsid
8. Enzyme-Linked Immunosorbent Assay USES SARS-CoV-2 IgG
Subject
Enzyme-Linked Immunosorbent Assay
Predicate
USES
Object
SARS-CoV-2 IgG
9. Serum LOCATION_OF immunoglobulin G
Subject
Serum
Predicate
LOCATION_OF
Object
immunoglobulin G
10. Antigens DISRUPTS Immune response
Subject
Antigens
Predicate
DISRUPTS
Object
Immune response
ABSTRACT

OBJECTIVES:

Specific serological tests are mandatory for reliable SARS-CoV-2 diagnostics and seroprevalence studies. Here, we assess the specificities of four commercially available SARS-CoV-2 IgG ELISAs in serum/plasma panels originating from Africa, South America, and Europe.

METHODS:

882 serum/plasma samples collected from symptom-free donors before the COVID-19 pandemic in three African countries (Ghana, Madagascar, Nigeria), Colombia, and Germany were analysed with three nucleocapsid-based ELISAs (Euroimmun Anti-SARS-CoV-2-NCP IgG, EDI™ Novel Coronavirus COVID-19 IgG, Mikrogen recomWell SARS-CoV-2 IgG), one spike/S1-based ELISA (Euroimmun Anti-SARS-CoV-2 IgG), and in-house common cold CoV ELISAs.

RESULTS:

High specificity was confirmed for all SARS-CoV-2 IgG ELISAs for Madagascan (93.4-99.4%), Colombian (97.8-100.0%), and German (95.9-100.0%) samples. In contrast, specificity was much lower for the Ghanaian and Nigerian serum panels (Ghana NCP-based assays 77.7-89.7%, spike/S1-based assay 94.3%; Nigeria NCP-based assays 39.3-82.7%, spike/S1-based assay 90.7%). 15 of 600 African sera were concordantly classified as positive in both the NCP-based and the spike/S1-based Euroimmun ELISA, but did not inhibit spike/ACE2 binding in a surrogate virus neutralisation test. IgG antibodies elicited by previous infections with common cold CoVs were found in all sample panels, including those from Madagascar, Colombia, and Germany and thus do not inevitably hamper assay specificity. Nevertheless, high levels of IgG antibodies interacting with OC43 NCP were found in all 15 SARS-CoV-2 NCP/spike/S1 ELISA positive sera.

CONCLUSIONS:

Depending on the chosen antigen and assay protocol, SARS-CoV-2 IgG ELISA specificity may be significantly reduced in certain populations probably due to interference of immune responses to endemic pathogens like other viruses or parasites.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / COVID-19 / Antibodies, Viral Subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Trop Med Int Health Clinical aspect: Diagnosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / COVID-19 / Antibodies, Viral Subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Trop Med Int Health Clinical aspect: Diagnosis Year: 2021
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