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Magnetization Transfer to Enhance NOE Cross-Peaks among Labile Protons: Applications to Imino-Imino Sequential Walks in SARS-CoV-2-Derived RNAs.
Novakovic, Mihajlo; Kupce, Eriks; Scherf, Tali; Oxenfarth, Andreas; Schnieders, Robbin; Grün, J Tassilo; Wirmer-Bartoschek, Julia; Richter, Christian; Schwalbe, Harald; Frydman, Lucio.
  • Novakovic M; Department of Chemical and Biological Physics, Weizmann Institute of Science, 7610001, Rehovot, Israel.
  • Kupce E; Bruker (UK) Ltd., Banner Lane, Coventry, UK.
  • Scherf T; Department of Chemical and Biological Physics, Weizmann Institute of Science, 7610001, Rehovot, Israel.
  • Oxenfarth A; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Schnieders R; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Grün JT; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Richter C; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University, 60438, Frankfurt/Main, Germany.
  • Frydman L; Department of Chemical and Biological Physics, Weizmann Institute of Science, 7610001, Rehovot, Israel.
Angew Chem Int Ed Engl ; 60(21): 11884-11891, 2021 05 17.
Article in English | MEDLINE | ID: covidwho-1121482
Semantic information from SemMedBD (by NLM)
1. Protons COEXISTS_WITH Nucleic Acids
Subject
Protons
Predicate
COEXISTS_WITH
Object
Nucleic Acids
2. Genome PART_OF 2019 novel coronavirus
Subject
Genome
Predicate
PART_OF
Object
2019 novel coronavirus
3. Protons COEXISTS_WITH Nucleic Acids
Subject
Protons
Predicate
COEXISTS_WITH
Object
Nucleic Acids
4. Genome PART_OF 2019 novel coronavirus
Subject
Genome
Predicate
PART_OF
Object
2019 novel coronavirus
ABSTRACT
2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent-driven exchanges to enhance NOE correlations between exchangeable and non-exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino-imino cross-peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS-CoV-2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Protons / RNA, Viral / Nuclear Magnetic Resonance, Biomolecular / SARS-CoV-2 Type of study: Randomized controlled trials Language: English Journal: Angew Chem Int Ed Engl Year: 2021 Document Type: Article Affiliation country: Anie.202015948

Full text: Available Collection: International databases Database: MEDLINE Main subject: Protons / RNA, Viral / Nuclear Magnetic Resonance, Biomolecular / SARS-CoV-2 Type of study: Randomized controlled trials Language: English Journal: Angew Chem Int Ed Engl Year: 2021 Document Type: Article Affiliation country: Anie.202015948