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Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing.
Zowawi, Hosam M; Alenazi, Thamer H; AlOmaim, Waleed S; Wazzan, Ahmad; Alsufayan, Abdullah; Hasanain, Rihab A; Aldibasi, Omar S; Althawadi, Sahar; Altamimi, Sarah A; Mutabagani, Maysoon; Alamri, Maha; Almaghrabi, Reem S; Al-Abdely, Hail M; Memish, Ziad A; Alqahtani, Saleh A.
  • Zowawi HM; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia zowawih@ksau-hs.edu.sa.
  • Alenazi TH; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia.
  • AlOmaim WS; UQ Centre for Clinical Research, Faculty of Medicine, University of Queensland, Brisbane Australia.
  • Wazzan A; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
  • Alsufayan A; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia.
  • Hasanain RA; Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
  • Aldibasi OS; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
  • Althawadi S; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia.
  • Altamimi SA; Prince Sultan Military Medical City, Ministry of Defence, Riyadh, Saudi Arabia.
  • Mutabagani M; IQVIA, Riyadh, Saudi Arabia.
  • Alamri M; College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
  • Almaghrabi RS; King Abdullah International Medical Research Centre, Riyadh, Saudi Arabia.
  • Al-Abdely HM; Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
  • Memish ZA; Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
  • Alqahtani SA; Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
J Clin Microbiol ; 59(5)2021 04 20.
Article in English | MEDLINE | ID: covidwho-1121790
ABSTRACT
Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on reverse transcriptase PCR (RT-PCR), which is routinely performed in well-equipped laboratories by trained professionals at specific locations. However, during busy periods, high numbers of samples queued for testing can delay the test results, impacting efforts to reduce the infection risk. Besides, the absence of well-established laboratories at remote sites and low-resourced environments can contribute to a silent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These reasons compel the need to accommodate point-of-care testing for COVID-19 that meets the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable). This study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests. Nasopharyngeal and nasal swabs were used. Of the 192 samples tested using the Biomeme SARS-CoV-2 system, the results from 189 samples (98.4%) were in agreement with the reference standard-of-care RT-PCR testing for SARS-CoV-2. The portable system generated simultaneous results for nine samples in 80 min with high positive and negative percent agreements of 99.0% and 97.8%, respectively. We performed separate testing in a sealed glove box, offering complete biosafety containment. Thus, the Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote and low-resourced settings.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / COVID-19 Nucleic Acid Testing / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: JCM.03004-20

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Reverse Transcriptase Polymerase Chain Reaction / COVID-19 Nucleic Acid Testing / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: JCM.03004-20