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The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019.
Fongaro, Gislaine; Stoco, Patrícia Hermes; Souza, Doris Sobral Marques; Grisard, Edmundo Carlos; Magri, Maria Elisa; Rogovski, Paula; Schörner, Marcos André; Barazzetti, Fernando Hartmann; Christoff, Ana Paula; de Oliveira, Luiz Felipe Valter; Bazzo, Maria Luiza; Wagner, Glauber; Hernández, Marta; Rodríguez-Lázaro, David.
  • Fongaro G; Applied Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Stoco PH; Protozoology Laboratory, Department of Microbiology, Imunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Souza DSM; Applied Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Grisard EC; Protozoology Laboratory, Department of Microbiology, Imunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Magri ME; Sanitary and Environmental Engineering Department, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Rogovski P; Applied Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Schörner MA; Molecular Biology, Microbiology and Serology Laboratory - LBMMS, University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Barazzetti FH; Molecular Biology, Microbiology and Serology Laboratory - LBMMS, University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Christoff AP; BiomeHub, Florianópolis, SC, Brazil.
  • de Oliveira LFV; Neoprospecta Microbiome Technologies, Florianópolis, SC, Brazil.
  • Bazzo ML; Molecular Biology, Microbiology and Serology Laboratory - LBMMS, University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Wagner G; Bioinformatics Laboratory, Departament of Microbiology, Imunology and Parasitology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Hernández M; Microbiology Division, Faculty of Sciences, University of Burgos, Burgos, Spain.
  • Rodríguez-Lázaro D; Microbiology Division, Faculty of Sciences, University of Burgos, Burgos, Spain. Electronic address: drlazaro@ubu.es.
Sci Total Environ ; 778: 146198, 2021 Jul 15.
Article in English | MEDLINE | ID: covidwho-1121857
Semantic information from SemMedBD (by NLM)
1. Laboratory LOCATION_OF Real-Time Polymerase Chain Reaction
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Laboratory
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LOCATION_OF
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Real-Time Polymerase Chain Reaction
2. 2019 novel coronavirus PROCESS_OF Community
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2019 novel coronavirus
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Community
3. Laboratory LOCATION_OF Real-Time Polymerase Chain Reaction
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Laboratory
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LOCATION_OF
Object
Real-Time Polymerase Chain Reaction
4. 2019 novel coronavirus PROCESS_OF Community
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2019 novel coronavirus
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Community
ABSTRACT
Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L-1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L-1, ranging from 5.49 ± 0.02 log10 GC L-1 (27th November 2019) to 6.68 ± 0.02 log10 GC L-1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Sci Total Environ Year: 2021 Document Type: Article Affiliation country: J.scitotenv.2021.146198

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Sci Total Environ Year: 2021 Document Type: Article Affiliation country: J.scitotenv.2021.146198