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SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis.
Bailey, Adam L; Dmytrenko, Oleksandr; Greenberg, Lina; Bredemeyer, Andrea L; Ma, Pan; Liu, Jing; Penna, Vinay; Winkler, Emma S; Sviben, Sanja; Brooks, Erin; Nair, Ajith P; Heck, Kent A; Rali, Aniket S; Simpson, Leo; Saririan, Mehrdad; Hobohm, Dan; Stump, W Tom; Fitzpatrick, James A; Xie, Xuping; Zhang, Xianwen; Shi, Pei-Yong; Hinson, J Travis; Gi, Weng-Tein; Schmidt, Constanze; Leuschner, Florian; Lin, Chieh-Yu; Diamond, Michael S; Greenberg, Michael J; Lavine, Kory J.
  • Bailey AL; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Dmytrenko O; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Greenberg L; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Bredemeyer AL; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Ma P; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Liu J; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Penna V; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Winkler ES; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Sviben S; Washington University Center for Cellular Imaging, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Brooks E; Department of Pathology & Laboratory Medicine, University of Wisconsin Hospital and Clinics, Madison, Wisconsin, USA.
  • Nair AP; Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
  • Heck KA; Department of Pathology, Baylor College of Medicine, Houston, Texas, USA.
  • Rali AS; Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
  • Simpson L; Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
  • Saririan M; Valleywise Health/Creighton University, Phoenix, Arizona, USA.
  • Hobohm D; Valleywise Health/Creighton University, Phoenix, Arizona, USA.
  • Stump WT; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Fitzpatrick JA; Washington University Center for Cellular Imaging, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Xie X; Departments of Neuroscience, Cell Biology & Physiology, and Biomedical Engineering, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Zhang X; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
  • Shi PY; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
  • Hinson JT; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
  • Gi WT; Departments of Cardiology, Genetics and Genome Sciences, UConn Health, Farmington, Connecticut, USA.
  • Schmidt C; The Jackson Laboratory for Genomic Medicine, Farmington, Connecticut, USA.
  • Leuschner F; Department of Internal Medicine III, University Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
  • Lin CY; Department of Internal Medicine III, University Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
  • Diamond MS; Department of Internal Medicine III, University Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
  • Greenberg MJ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
  • Lavine KJ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
JACC Basic Transl Sci ; 6(4): 331-345, 2021 Apr.
Article in English | MEDLINE | ID: covidwho-1122096
ABSTRACT
There is ongoing debate as to whether cardiac complications of coronavirus disease-2019 (COVID-19) result from myocardial viral infection or are secondary to systemic inflammation and/or thrombosis. We provide evidence that cardiomyocytes are infected in patients with COVID-19 myocarditis and are susceptible to severe acute respiratory syndrome coronavirus 2. We establish an engineered heart tissue model of COVID-19 myocardial pathology, define mechanisms of viral pathogenesis, and demonstrate that cardiomyocyte severe acute respiratory syndrome coronavirus 2 infection results in contractile deficits, cytokine production, sarcomere disassembly, and cell death. These findings implicate direct infection of cardiomyocytes in the pathogenesis of COVID-19 myocardial pathology and provides a model system to study this emerging disease.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: JACC Basic Transl Sci Year: 2021 Document Type: Article Affiliation country: J.jacbts.2021.01.002

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: JACC Basic Transl Sci Year: 2021 Document Type: Article Affiliation country: J.jacbts.2021.01.002