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Arginase 1 (Arg1) as an Up-Regulated Gene in COVID-19 Patients: A Promising Marker in COVID-19 Immunopathy.
Derakhshani, Afshin; Hemmat, Nima; Asadzadeh, Zahra; Ghaseminia, Moslem; Shadbad, Mahdi Abdoli; Jadideslam, Golamreza; Silvestris, Nicola; Racanelli, Vito; Baradaran, Behzad.
  • Derakhshani A; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
  • Hemmat N; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
  • Asadzadeh Z; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
  • Ghaseminia M; Department of Virology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz 5166614766, Iran.
  • Shadbad MA; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran.
  • Jadideslam G; Student Research Committee, Tabriz University of Medical Sciences, Tabriz 5166614766, Iran.
  • Silvestris N; Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz 5166614766, Iran.
  • Racanelli V; IRCCS Istituto Tumori "Giovanni Paolo II" of Bari, 70124 Bari, Italy.
  • Baradaran B; Department of Biomedical Sciences and Human Oncology (DIMO), University of Bari, 70124 Bari, Italy.
J Clin Med ; 10(5)2021 Mar 04.
Article in English | MEDLINE | ID: covidwho-1124853
Semantic information from SemMedBD (by NLM)
1. ARG1 gene|ARG1 STIMULATES Genes
Subject
ARG1 gene|ARG1
Predicate
STIMULATES
Object
Genes
2. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
3. Patients LOCATION_OF Genes
Subject
Patients
Predicate
LOCATION_OF
Object
Genes
4. COVID-19 AFFECTS Immune response
Subject
COVID-19
Predicate
AFFECTS
Object
Immune response
5. ARG1 gene|ARG1 INTERACTS_WITH Immune Cell
Subject
ARG1 gene|ARG1
Predicate
INTERACTS_WITH
Object
Immune Cell
6. Real-Time Polymerase Chain Reaction MEASURES Anabolism
Subject
Real-Time Polymerase Chain Reaction
Predicate
MEASURES
Object
Anabolism
7. ARG1 gene|ARG1 LOCATION_OF Gene Expression
Subject
ARG1 gene|ARG1
Predicate
LOCATION_OF
Object
Gene Expression
8. Patients LOCATION_OF ARG1 gene|ARG1
Subject
Patients
Predicate
LOCATION_OF
Object
ARG1 gene|ARG1
9. peripheral blood PART_OF Patients
Subject
peripheral blood
Predicate
PART_OF
Object
Patients
10. ARG1 gene|ARG1 CAUSES Disease
Subject
ARG1 gene|ARG1
Predicate
CAUSES
Object
Disease
11. ARG1 gene|ARG1 STIMULATES Genes
Subject
ARG1 gene|ARG1
Predicate
STIMULATES
Object
Genes
12. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
13. Patients LOCATION_OF Genes
Subject
Patients
Predicate
LOCATION_OF
Object
Genes
14. COVID-19 AFFECTS Immune response
Subject
COVID-19
Predicate
AFFECTS
Object
Immune response
15. ARG1 gene|ARG1 INTERACTS_WITH Immune Cell
Subject
ARG1 gene|ARG1
Predicate
INTERACTS_WITH
Object
Immune Cell
16. Real-Time Polymerase Chain Reaction MEASURES Anabolism
Subject
Real-Time Polymerase Chain Reaction
Predicate
MEASURES
Object
Anabolism
17. ARG1 gene|ARG1 LOCATION_OF Gene Expression
Subject
ARG1 gene|ARG1
Predicate
LOCATION_OF
Object
Gene Expression
18. Patients LOCATION_OF ARG1 gene|ARG1
Subject
Patients
Predicate
LOCATION_OF
Object
ARG1 gene|ARG1
19. peripheral blood PART_OF Patients
Subject
peripheral blood
Predicate
PART_OF
Object
Patients
20. ARG1 gene|ARG1 CAUSES Disease
Subject
ARG1 gene|ARG1
Predicate
CAUSES
Object
Disease
ABSTRACT

BACKGROUND:

The coronavirus disease 2019 (COVID-19) outbreak, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been declared a global pandemic. It is well-established that SARS-CoV-2 infection can lead to dysregulated immune responses. Arginase-1 (Arg1), which has a pivotal role in immune cells, can be expressed in most of the myeloid cells, e.g., neutrophils and macrophages. Arg1 has been associated with the suppression of antiviral immune responses.

METHODS:

Whole blood was taken from 21 COVID-19 patients and 21 healthy individuals, and after RNA extraction and complementary DNA (cDNA) synthesis, gene expression of Arg1 was measured by real-time PCR.

RESULTS:

The qPCR results showed that the expression of Arg1 was significantly increased in COVID-19 patients compared to healthy individuals (p < 0.01). The relative expression analysis demonstrated there were approximately 2.3 times increased Arg1 expression in the whole blood of COVID-19 patients. Furthermore, the receiver operating characteristic (ROC) analysis showed a considerable diagnostic value for Arg1 expression in COVID-19 (p = 0.0002 and AUC = 0.8401).

CONCLUSION:

Arg1 might be a promising marker in the pathogenesis of the disease, and it could be a valuable diagnostic tool.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Jcm10051051

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Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Jcm10051051