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Comparative Assessment of Sera from Individuals after S-Gene RNA-Based SARS-CoV-2 Vaccination with Spike-Protein-Based and Nucleocapsid-Based Serological Assays.
Dörschug, Anja; Frickmann, Hagen; Schwanbeck, Julian; Yilmaz, Elif; Mese, Kemal; Hahn, Andreas; Groß, Uwe; Zautner, Andreas E.
  • Dörschug A; Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Frickmann H; Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany.
  • Schwanbeck J; Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany.
  • Yilmaz E; Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Mese K; Department of Anesthesiology, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Hahn A; Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.
  • Groß U; Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany.
  • Zautner AE; Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.
Diagnostics (Basel) ; 11(3)2021 Mar 03.
Article in English | MEDLINE | ID: covidwho-1125041
ABSTRACT
Due to the beginning of vaccination against COVID-19, serological discrimination between vaccine-associated humoral response and serology-based surveillance of natural SARS-CoV-2 infections as well as breakthrough infections becomes an issue of relevance. Here, we assessed the differentiated effects of the application of an RNA vaccine using SARS-CoV-2 spike protein epitopes on the results of both anti-spike protein-based serology (EUROIMMUN) and anti-nucleocapsid-based serology (VIROTECH). A total of 80 serum samples from vaccinees acquired at different time points after vaccination was assessed. While positive or borderline serological response in the anti-spike protein assay was observed for all samples (90% both IgG and IgA, 6.3% IgA only, 3.8% borderline IgG only), only a single case of a falsely positive IgM was observed for the anti-nucleocapsid assay as expected due to this assay's specificity. Positive anti-spike protein antibodies were already detectable in the second week after the first dose of vaccination, with higher titers after the second dose of the vaccine. In conclusion, the combined application of anti-spike protein-based serology and anti-nucleocapsid-based serology will provide a useful option for the discrimination of vaccination response and natural infection.
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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Diagnostics11030426

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Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Diagnostics11030426