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Blockade of SARS-CoV-2 spike protein-mediated cell-cell fusion using COVID-19 convalescent plasma.
Wang, Ling; Zhao, Juan; Nguyen, Lam N T; Adkins, James L; Schank, Madison; Khanal, Sushant; Nguyen, Lam N; Dang, Xindi; Cao, Dechao; Thakuri, Bal Krishna Chand; Lu, Zeyuan; Zhang, Jinyu; Zhang, Yi; Wu, Xiao Y; El Gazzar, Mohamed; Ning, Shunbin; Moorman, Jonathan P; Yao, Zhi Q.
  • Wang L; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Zhao J; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Nguyen LNT; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Adkins JL; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Schank M; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Khanal S; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Nguyen LN; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Dang X; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Cao D; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Thakuri BKC; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Lu Z; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Zhang J; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Zhang Y; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Wu XY; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • El Gazzar M; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Ning S; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
  • Moorman JP; Division of Infectious, Inflammatory and Immunologic Diseases, Department of Internal Medicine, Quillen College of Medicine, ETSU, Johnson City, TN, 37614, USA.
  • Yao ZQ; Center of Excellence for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, 37614, USA.
Sci Rep ; 11(1): 5558, 2021 03 10.
Article in English | MEDLINE | ID: covidwho-1125779
Semantic information from SemMedBD (by NLM)
1. spike protei AFFECTS C1261468
Subject
spike protei
Predicate
AFFECTS
Object
C1261468
2. Vitronecti AFFECTS C1537068
Subject
Vitronecti
Predicate
AFFECTS
Object
C1537068
3. COVID-19 convalescent plasma PART_OF Homo sapiens
Subject
COVID-19 convalescent plasma
Predicate
PART_OF
Object
Homo sapiens
4. spike protei ADMINISTERED_TO C0221284
Subject
spike protei
Predicate
ADMINISTERED_TO
Object
C0221284
5. Antibodie AFFECTS C1261468
Subject
Antibodie
Predicate
AFFECTS
Object
C1261468
6. Antibodie AFFECTS C1537068
Subject
Antibodie
Predicate
AFFECTS
Object
C1537068
7. spike protein, SARS-CoV-2 AFFECTS Cell fusion
Subject
spike protein, SARS-CoV-2
Predicate
AFFECTS
Object
Cell fusion
8. Vitronectin, human AFFECTS Virus Internalization
Subject
Vitronectin, human
Predicate
AFFECTS
Object
Virus Internalization
9. COVID-19 convalescent plasma PART_OF Homo sapiens
Subject
COVID-19 convalescent plasma
Predicate
PART_OF
Object
Homo sapiens
10. spike protein, SARS-CoV-2 ADMINISTERED_TO Leptocyte
Subject
spike protein, SARS-CoV-2
Predicate
ADMINISTERED_TO
Object
Leptocyte
11. Antibodies, Neutralizing AFFECTS Cell fusion
Subject
Antibodies, Neutralizing
Predicate
AFFECTS
Object
Cell fusion
12. Antibodies, Neutralizing AFFECTS Virus Internalization
Subject
Antibodies, Neutralizing
Predicate
AFFECTS
Object
Virus Internalization
ABSTRACT
The recent COVID-19 pandemic poses a serious threat to global public health, thus there is an urgent need to define the molecular mechanisms involved in SARS-CoV-2 spike (S) protein-mediated virus entry that is essential for preventing and/or treating this emerging infectious disease. In this study, we examined the blocking activity of human COVID-19 convalescent plasma by cell-cell fusion assays using SARS-CoV-2-S-transfected 293 T as effector cells and ACE2-expressing 293 T as target cells. We demonstrate that the SARS-CoV-2 S protein exhibits a very high capacity for membrane fusion and is efficient in mediating virus fusion and entry into target cells. Importantly, we find that COVID-19 convalescent plasma with high titers of IgG neutralizing antibodies can block cell-cell fusion and virus entry by interfering with the SARS-CoV-2-S/ACE2 or SARS-CoV-S/ACE2 interactions. These findings suggest that COVID-19 convalescent plasma may not only inhibit SARS-CoV-2-S but also cross-neutralize SARS-CoV-S-mediated membrane fusion and virus entry, supporting its potential as a preventive and/or therapeutic agent against SARS-CoV-2 as well as other SARS-CoV infections.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-84840-3

Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-84840-3