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Identification of the absorbed ingredients and metabolites in rats after an intravenous administration of Tanreqing injection using high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
Xie, Like; Wang, Yangyang; Yin, Hao; Li, Jiajia; Xu, Zhou; Sun, Zhaolin; Liu, Fang; Zhang, Xiaoli; Liu, Shaoyong; Sun, Jianguo; Tian, Xiaoting; Huang, Chenggang.
  • Xie L; Key Lab of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu, P. R. China.
  • Wang Y; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Yin H; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Li J; University of Chinese Academy of Sciences, Beijing, P. R. China.
  • Xu Z; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Sun Z; University of Chinese Academy of Sciences, Beijing, P. R. China.
  • Liu F; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Zhang X; University of Chinese Academy of Sciences, Beijing, P. R. China.
  • Liu S; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Sun J; University of Chinese Academy of Sciences, Beijing, P. R. China.
  • Tian X; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China.
  • Huang C; University of Chinese Academy of Sciences, Beijing, P. R. China.
J Sep Sci ; 44(10): 2097-2112, 2021 May.
Article in English | MEDLINE | ID: covidwho-1130643
ABSTRACT
The metabolic profiles of Tanreqing injection, which is a traditional Chinese medicine recommended for complementary administration to treat a novel coronavirus, have remained unclear, which inhibit the understanding of the effective chemical compounds of Tanreqing injection. In this study, a sensitive high-performance liquid chromatography quadrupole time-of-flight mass spectrometry method was used to identify the compounds and metabolites in various biosamples, including plasma, bile, liver, lung, kidney, urine, and feces, following the intravenous administration of Tanreqing injection in rats. A total of 89 compounds were characterized in the biosamples of Tanreqing injection-treated rats including 25 precursor constituents and 64 metabolites. Nine flavonoid compounds, twelve phenolic acids, and four iridoid glycosides were identified in the rats. Their metabolites were mainly produced by glucuronidation, deglucuronidation, glycosylation, deglycosylation, methylation, demethylation, N-heterocyclisation, sulphation, dehydroxylation, decarboxylation, dehydration, hydroxylation, and corresponding recombination reactions. This study was the first to comprehensively investigate the metabolic profile of Tanreqing injection and provides a scientific basis to further elucidate the pharmacodynamic material basis and therapeutic mechanism of Tanreqing injection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Chromatography, High Pressure Liquid / Tandem Mass Spectrometry Type of study: Diagnostic study Topics: Traditional medicine Limits: Animals Language: English Journal: J Sep Sci Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Chromatography, High Pressure Liquid / Tandem Mass Spectrometry Type of study: Diagnostic study Topics: Traditional medicine Limits: Animals Language: English Journal: J Sep Sci Year: 2021 Document Type: Article