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Comprehensive analysis of SARS-CoV-2 antibody dynamics in New Zealand.
Whitcombe, Alana L; McGregor, Reuben; Craigie, Alyson; James, Alex; Charlewood, Richard; Lorenz, Natalie; Dickson, James Mj; Sheen, Campbell R; Koch, Barbara; Fox-Lewis, Shivani; McAuliffe, Gary; Roberts, Sally A; Morpeth, Susan C; Taylor, Susan; Webb, Rachel H; Jack, Susan; Upton, Arlo; Ussher, James E; Moreland, Nicole J.
  • Whitcombe AL; Faculty of Medical and Health Sciences University of Auckland Auckland New Zealand.
  • McGregor R; Maurice Wilkins Centre University of Auckland Auckland New Zealand.
  • Craigie A; Faculty of Medical and Health Sciences University of Auckland Auckland New Zealand.
  • James A; Maurice Wilkins Centre University of Auckland Auckland New Zealand.
  • Charlewood R; Southern Community Laboratories Dunedin New Zealand.
  • Lorenz N; Te Punaha Matatini and School of Mathematics and Statistics University of Canterbury Christchurch New Zealand.
  • Dickson JM; New Zealand Blood Service Auckland New Zealand.
  • Sheen CR; Faculty of Medical and Health Sciences University of Auckland Auckland New Zealand.
  • Koch B; Maurice Wilkins Centre University of Auckland Auckland New Zealand.
  • Fox-Lewis S; School of Biological Sciences University of Auckland Auckland New Zealand.
  • McAuliffe G; Protein Science and Engineering Callaghan Innovation Christchurch New Zealand.
  • Roberts SA; Protein Science and Engineering Callaghan Innovation Christchurch New Zealand.
  • Morpeth SC; LabPLUS Auckland City Hospital Auckland New Zealand.
  • Taylor S; LabPLUS Auckland City Hospital Auckland New Zealand.
  • Webb RH; Maurice Wilkins Centre University of Auckland Auckland New Zealand.
  • Jack S; LabPLUS Auckland City Hospital Auckland New Zealand.
  • Upton A; Middlemore Hospital Auckland New Zealand.
  • Ussher JE; Middlemore Hospital Auckland New Zealand.
  • Moreland NJ; Faculty of Medical and Health Sciences University of Auckland Auckland New Zealand.
Clin Transl Immunology ; 10(3): e1261, 2021.
Article in English | MEDLINE | ID: covidwho-1135089
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT

OBJECTIVES:

Circulating antibodies are important markers of previous infection and immunity. Questions remain with respect to the durability and functionality of SARS-CoV-2 antibodies. This study explored antibody responses in recovered COVID-19 patients in a setting where the probability of re-exposure is effectively nil, owing to New Zealand's successful elimination strategy.

METHODS:

A triplex bead-based assay that detects antibody isotype (IgG, IgM and IgA) and subclass (IgG1, IgG2, IgG3 and IgG4) responses against Nucleocapsid (N) protein, the receptor binding domain (RBD) and Spike (S) protein of SARS-CoV-2 was developed. After establishing baseline levels with pre-pandemic control sera (n = 113), samples from PCR-confirmed COVID-19 patients with mild-moderate disease (n = 189) collected up to 8 months post-infection were examined. The relationship between antigen-specific antibodies and neutralising antibodies (NAbs) was explored with a surrogate neutralisation assay that quantifies inhibition of the RBD/hACE-2 interaction.

RESULTS:

While most individuals had broad isotype and subclass responses to each antigen shortly after infection, only RBD and S protein IgG, as well as NAbs, were relatively stable over the study period, with 99%, 96% and 90% of samples, respectively, having responses over baseline 4-8 months post-infection. Anti-RBD antibodies were strongly correlated with NAbs at all time points (Pearson's r ≥ 0.87), and feasibility of using finger prick sampling to accurately measure anti-RBD IgG was demonstrated.

CONCLUSION:

Antibodies to SARS-CoV-2 persist for up to 8 months following mild-to-moderate infection. This robust response can be attributed to the initial exposure without immune boosting given the lack of community transmission in our setting.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Clin Transl Immunology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Clin Transl Immunology Year: 2021 Document Type: Article