Your browser doesn't support javascript.
Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2.
Sundah, Noah R; Natalia, Auginia; Liu, Yu; Ho, Nicholas R Y; Zhao, Haitao; Chen, Yuan; Miow, Qing Hao; Wang, Yu; Beh, Darius L L; Chew, Ka Lip; Chan, Douglas; Tambyah, Paul A; Ong, Catherine W M; Shao, Huilin.
  • Sundah NR; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Natalia A; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
  • Liu Y; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Ho NRY; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
  • Zhao H; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Chen Y; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
  • Miow QH; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Wang Y; Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore, Singapore.
  • Beh DLL; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Chew KL; Institute for Health Innovation&Technology, National University of Singapore, Singapore, Singapore.
  • Chan D; Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
  • Tambyah PA; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Ong CWM; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
  • Shao H; Division of Infectious Diseases, Department of Medicine, National University Hospital, Singapore, Singapore.
Sci Adv ; 7(12)2021 03.
Article in English | MEDLINE | ID: covidwho-1140315
ABSTRACT
Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state-the transition state-that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (~8 RNA copies/µl), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Microfluidic Analytical Techniques / Lab-On-A-Chip Devices / Point-of-Care Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Sciadv.abe5940

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Microfluidic Analytical Techniques / Lab-On-A-Chip Devices / Point-of-Care Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Sciadv.abe5940