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A luciferase immunosorbent assay for quantitative detection of IgG antibodies against SARS-CoV-2 nucleoprotein.
Liang, Yuanhao; Yan, Huanchang; Huang, Liping; Zhao, Jianhui; Wang, Haiying; Kang, Min; Wan, Zhengwei; Shui, Jingwei; Tang, Shixing.
  • Liang Y; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Yan H; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Huang L; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Zhao J; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Wang H; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Kang M; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Wan Z; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Shui J; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
  • Tang S; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China; Hospital of Dermatology, Southern Medical University, Guangzhou, 510515, China. Electronic address: tamgshixing@smu.edu.cn.
J Virol Methods ; 292: 114141, 2021 06.
Article in English | MEDLINE | ID: covidwho-1142107
Semantic information from SemMedBD (by NLM)
1. Nucleoproteins PART_OF 2019 novel coronavirus
Subject
Nucleoproteins
Predicate
PART_OF
Object
2019 novel coronavirus
2. Assay USES Immunosorbents
Subject
Assay
Predicate
USES
Object
Immunosorbents
3. Assay METHOD_OF Detection
Subject
Assay
Predicate
METHOD_OF
Object
Detection
4. Antibodie PRODUCES C0024075
Subject
Antibodie
Predicate
PRODUCES
Object
C0024075
5. Serum LOCATION_OF Antibodie
Subject
Serum
Predicate
LOCATION_OF
Object
Antibodie
6. Plasma specimen LOCATION_OF Antibodie
Subject
Plasma specimen
Predicate
LOCATION_OF
Object
Antibodie
7. Nucleoproteins PART_OF SARS coronavirus
Subject
Nucleoproteins
Predicate
PART_OF
Object
SARS coronavirus
8. Monoclonal Antibodies PART_OF Mus
Subject
Monoclonal Antibodies
Predicate
PART_OF
Object
Mus
9. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
10. Nucleoproteins PART_OF 2019 novel coronavirus
Subject
Nucleoproteins
Predicate
PART_OF
Object
2019 novel coronavirus
11. Assay USES Immunosorbents
Subject
Assay
Predicate
USES
Object
Immunosorbents
12. Assay METHOD_OF Detection
Subject
Assay
Predicate
METHOD_OF
Object
Detection
13. Antibodies, Anti-Idiotypic PRODUCES Luciferases
Subject
Antibodies, Anti-Idiotypic
Predicate
PRODUCES
Object
Luciferases
14. Serum LOCATION_OF Antibodies, Anti-Idiotypic
Subject
Serum
Predicate
LOCATION_OF
Object
Antibodies, Anti-Idiotypic
15. Plasma specimen LOCATION_OF Antibodies, Anti-Idiotypic
Subject
Plasma specimen
Predicate
LOCATION_OF
Object
Antibodies, Anti-Idiotypic
16. Nucleoproteins PART_OF SARS coronavirus
Subject
Nucleoproteins
Predicate
PART_OF
Object
SARS coronavirus
17. Monoclonal Antibodies PART_OF Mus
Subject
Monoclonal Antibodies
Predicate
PART_OF
Object
Mus
18. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
ABSTRACT
In this study, we developed and evaluated a luciferase immunosorbent assay (LISA) for quantitative detection of IgG antibody against SARS-CoV-2 nucleoprotein (NP). Anti-SARS-CoV-2 NP antibody in serum or plasma samples was captured by protein G-coated microtiter plate and detected using the crude cell lysates expressing Nanoluc luciferase (Nluc) enzyme fused with SARS-CoV-2 NP. After the addition of furimazine substrate, the levels of anti-SARS-CoV-2 NP IgG antibody were quantitatively measured as luciferase light units. As expected, SARS-CoV-2 NP showed cross-reactivity with the monoclonal antibodies against SARS-CoV NP, but not MERS-CoV NP-specific monoclonal antibodies or the monoclonal antibodies against SARS-CoV Spike protein. LISA for detecting murine monoclonal antibody against SARS-CoV NP showed a low limit of detection of 0.4 pg/µl and linear detection range from 0.4 pg/µl to 75 pg/µl. Furthermore, LISA had a sensitivity of 71 % when testing COVID-19 patients at the second week post onset and a specificity of 100 % when testing healthy blood donors.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: J Virol Methods Clinical aspect: Diagnosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / Nucleocapsid Proteins / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: J Virol Methods Clinical aspect: Diagnosis Year: 2021
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