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Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses.
Li, Zeshi; Lang, Yifei; Liu, Lin; Bunyatov, Mehman I; Sarmiento, Angelic Isaza; de Groot, Raoul J; Boons, Geert-Jan.
  • Li Z; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
  • Lang Y; Virology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • Liu L; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
  • Bunyatov MI; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
  • Sarmiento AI; Virology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • de Groot RJ; Virology Division, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. r.j.degroot@uu.nl.
  • Boons GJ; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. g.j.p.h.boons@uu.nl.
Nat Chem ; 13(5): 496-503, 2021 05.
Article in English | MEDLINE | ID: covidwho-1145994
ABSTRACT
The transmission of viruses from animal reservoirs to humans poses major threats to public health. Preparedness for future zoonotic outbreaks requires a fundamental understanding of how viruses of animal origin have adapted to binding to a cell surface component and/or receptor of the new host. Here we report on the specificities of human and animal viruses that engage with O-acetylated sialic acid, which include betacoronaviruses, toroviruses and influenza C and D viruses. Key to these studies was the development of a chemoenzymatic methodology that can provide almost any sialate-acetylation pattern. A collection of O-acetylated sialoglycans was printed as a microarray for the determination of receptor specificity. These studies showed host-specific patterns of receptor recognition and revealed that three distinct human respiratory viruses uniquely bind 9-O-acetylated α2,8-linked disialoside. Immunofluorescence and cell entry studies support that such a glycotope as part of a ganglioside is a functional receptor for human coronaviruses.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Tract Infections / Viruses / N-Acetylneuraminic Acid Limits: Humans Language: English Journal: Nat Chem Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: S41557-021-00655-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Tract Infections / Viruses / N-Acetylneuraminic Acid Limits: Humans Language: English Journal: Nat Chem Journal subject: Chemistry Year: 2021 Document Type: Article Affiliation country: S41557-021-00655-9