A tissue- and gender-specific regulation of the SARS-CoV-2 receptor ACE2 by p53 in pigs.
Biochem Biophys Res Commun
; 553: 25-29, 2021 05 14.
Article
in English
| MEDLINE | ID: covidwho-1147359
ABSTRACT
The current COVID-19 pandemic is caused by infections with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A sex-bias has been observed, with increased susceptibility and mortality in male compared to female patients. The gene for the SARS-CoV-2 receptor ACE2 is located on the X chromosome. We previously generated TP53 mutant pigs that exhibit a sex-specific patho-phenotype due to altered regulation of numerous X chromosome genes. In this study, we explored the effect of p53 deficiency on ACE2 expression in pigs. First, we identified the p53 binding site in the ACE2 promoter and could show its regulatory effect on ACE2 expression by luciferase assay in porcine primary kidney fibroblast cells. Later, quantitative PCR and western blot showed tissue- and gender-specific expression changes of ACE2 and its truncated isoform in p53-deficient pigs. We believe these findings will broaden the knowledge on ACE2 regulation and COVID-19 susceptibility.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Organ Specificity
/
Gene Expression Regulation
/
Tumor Suppressor Protein p53
/
Sex Characteristics
/
Sus scrofa
/
Angiotensin-Converting Enzyme 2
Limits:
Animals
Language:
English
Journal:
Biochem Biophys Res Commun
Year:
2021
Document Type:
Article
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