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A tissue- and gender-specific regulation of the SARS-CoV-2 receptor ACE2 by p53 in pigs.
Zhang, Yue; Niu, Guanglin; Flisikowska, Tatiana; Schnieke, Angelika; Flisikowski, Krzysztof.
  • Zhang Y; Livestock Biotechnology, School of Life Science, Technische Universität München, Germany. Electronic address: yuezhang@wzw.tum.de.
  • Niu G; Livestock Biotechnology, School of Life Science, Technische Universität München, Germany. Electronic address: niu@wzw.tum.de.
  • Flisikowska T; Livestock Biotechnology, School of Life Science, Technische Universität München, Germany. Electronic address: tatiana.flisikowska@wzw.tum.de.
  • Schnieke A; Livestock Biotechnology, School of Life Science, Technische Universität München, Germany. Electronic address: schnieke@wzw.tum.de.
  • Flisikowski K; Livestock Biotechnology, School of Life Science, Technische Universität München, Germany. Electronic address: flisikowski@wzw.tum.de.
Biochem Biophys Res Commun ; 553: 25-29, 2021 05 14.
Article in English | MEDLINE | ID: covidwho-1147359
Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus PROCESS_OF Family suidae
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
Family suidae
2. Genes PART_OF X Chromosome
Subject
Genes
Predicate
PART_OF
Object
X Chromosome
3. TP53 INTERACTS_WITH Gene
Subject
TP53
Predicate
INTERACTS_WITH
Object
Gene
4. Family suidae LOCATION_OF ACE2 gene|ACE2
Subject
Family suidae
Predicate
LOCATION_OF
Object
ACE2 gene|ACE2
5. TP53 gene|TP53 INTERACTS_WITH ACE2 gene|ACE2
Subject
TP53 gene|TP53
Predicate
INTERACTS_WITH
Object
ACE2 gene|ACE2
6. Assay USES Luciferases
Subject
Assay
Predicate
USES
Object
Luciferases
7. Epithelial cell of renal tubule LOCATION_OF ACE2 gene|ACE2
Subject
Epithelial cell of renal tubule
Predicate
LOCATION_OF
Object
ACE2 gene|ACE2
8. 2019 novel coronavirus PROCESS_OF Family suidae
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
Family suidae
9. Genes PART_OF X Chromosome
Subject
Genes
Predicate
PART_OF
Object
X Chromosome
10. TP53 INTERACTS_WITH Genes, X-Linked
Subject
TP53
Predicate
INTERACTS_WITH
Object
Genes, X-Linked
11. Family suidae LOCATION_OF ACE2 gene|ACE2
Subject
Family suidae
Predicate
LOCATION_OF
Object
ACE2 gene|ACE2
12. TP53 gene|TP53 INTERACTS_WITH ACE2 gene|ACE2
Subject
TP53 gene|TP53
Predicate
INTERACTS_WITH
Object
ACE2 gene|ACE2
13. Assay USES Luciferases
Subject
Assay
Predicate
USES
Object
Luciferases
14. Epithelial cell of renal tubule LOCATION_OF ACE2 gene|ACE2
Subject
Epithelial cell of renal tubule
Predicate
LOCATION_OF
Object
ACE2 gene|ACE2
ABSTRACT
The current COVID-19 pandemic is caused by infections with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A sex-bias has been observed, with increased susceptibility and mortality in male compared to female patients. The gene for the SARS-CoV-2 receptor ACE2 is located on the X chromosome. We previously generated TP53 mutant pigs that exhibit a sex-specific patho-phenotype due to altered regulation of numerous X chromosome genes. In this study, we explored the effect of p53 deficiency on ACE2 expression in pigs. First, we identified the p53 binding site in the ACE2 promoter and could show its regulatory effect on ACE2 expression by luciferase assay in porcine primary kidney fibroblast cells. Later, quantitative PCR and western blot showed tissue- and gender-specific expression changes of ACE2 and its truncated isoform in p53-deficient pigs. We believe these findings will broaden the knowledge on ACE2 regulation and COVID-19 susceptibility.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Organ Specificity / Gene Expression Regulation / Tumor Suppressor Protein p53 / Sex Characteristics / Sus scrofa / Angiotensin-Converting Enzyme 2 Subject: Organ Specificity / Gene Expression Regulation / Tumor Suppressor Protein p53 / Sex Characteristics / Sus scrofa / Angiotensin-Converting Enzyme 2 Type of study: Determinantes_sociais_saude / Prognostic study Language: English Journal: Biochem Biophys Res Commun Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Organ Specificity / Gene Expression Regulation / Tumor Suppressor Protein p53 / Sex Characteristics / Sus scrofa / Angiotensin-Converting Enzyme 2 Subject: Organ Specificity / Gene Expression Regulation / Tumor Suppressor Protein p53 / Sex Characteristics / Sus scrofa / Angiotensin-Converting Enzyme 2 Type of study: Determinantes_sociais_saude / Prognostic study Language: English Journal: Biochem Biophys Res Commun Year: 2021
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