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Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.
Elledge, Susanna K; Zhou, Xin X; Byrnes, James R; Martinko, Alexander J; Lui, Irene; Pance, Katarina; Lim, Shion A; Glasgow, Jeff E; Glasgow, Anum A; Turcios, Keirstinne; Iyer, Nikita S; Torres, Leonel; Peluso, Michael J; Henrich, Timothy J; Wang, Taia T; Tato, Cristina M; Leung, Kevin K; Greenhouse, Bryan; Wells, James A.
  • Elledge SK; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Zhou XX; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Byrnes JR; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Martinko AJ; Soteria Biotherapeutics, San Francisco, CA, USA.
  • Lui I; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Pance K; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Lim SA; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Glasgow JE; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
  • Glasgow AA; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA.
  • Turcios K; Division of Experimental Medicine, University of California, San Francisco, San Francisco CA, USA.
  • Iyer NS; Division of Experimental Medicine, University of California, San Francisco, San Francisco CA, USA.
  • Torres L; Division of Experimental Medicine, University of California, San Francisco, San Francisco CA, USA.
  • Peluso MJ; Division of HIV, Infectious Diseases, and Global Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Henrich TJ; Division of HIV, Infectious Diseases, and Global Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Wang TT; Division of Experimental Medicine, University of California, San Francisco, San Francisco CA, USA.
  • Tato CM; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Leung KK; Departments of Medicine and of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
  • Greenhouse B; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Wells JA; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Nat Biotechnol ; 39(8): 928-935, 2021 08.
Article in English | MEDLINE | ID: covidwho-1152862
Preprint
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ABSTRACT
Current serology tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies mainly take the form of enzyme-linked immunosorbent assays, chemiluminescent microparticle immunoassays or lateral flow assays, which are either laborious, expensive or lacking sufficient sensitivity and scalability. Here we present the development and validation of a rapid, low-cost, solution-based assay to detect antibodies in serum, plasma, whole blood and to a lesser extent saliva, using rationally designed split luciferase antibody biosensors. This new assay, which generates quantitative results in 30 min, substantially reduces the complexity and improves the scalability of coronavirus disease 2019 (COVID-19) antibody tests. This assay is well-suited for point-of-care, broad population testing, and applications in low-resource settings, for monitoring host humoral responses to vaccination or viral infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Point-of-Care Systems / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: S41587-021-00878-8

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Point-of-Care Systems / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: Nat Biotechnol Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: S41587-021-00878-8