Rapid and ultrasensitive method for determination of aflatoxin M1 in milk
Food and Agricultural Immunology
; 31(1):849-858, 2020.
Article
in English
| CAB Abstracts | ID: covidwho-1152988
ABSTRACT
Aflatoxin M1 (AFM1) in milk is a problem of great concern. Current methods of detection require large instruments and need specific test sites. Therefore, it is necessary to establish a fast, convenient, and accurate detection method for AFM1. We established a system based on fluorescent microspheres containing a Eu<sup>3+</sup> chelate named AFM1-POCT. These components comprised the AFM1-POCT kits. After refrigeration at 4 degrees C for 12 months, the intra and inter assay coefficients of variability (CVs) for the kits were 4% and 5%, respectively. AFM1-POCT compared well with ultra-high-performance liquid chromatography (UHPLC) determination in the range 0.0121-2 g/kg (paired samples test, P > 0.05). Aflatoxin B1 and G1 do not react with aflatoxin M1. By using the AFM1-POCT method, the detection time is shortened to 5 min, the accuracy is comparable to that of UHPLC, and convenience and range of application are improved.
Full text:
Available
Collection:
Databases of international organizations
Database:
CAB Abstracts
Language:
English
Journal:
Food and Agricultural Immunology
Year:
2020
Document Type:
Article
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