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A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material.
Rauch, Jennifer N; Valois, Eric; Solley, Sabrina C; Braig, Friederike; Lach, Ryan S; Audouard, Morgane; Ponce-Rojas, Jose Carlos; Costello, Michael S; Baxter, Naomi J; Kosik, Kenneth S; Arias, Carolina; Acosta-Alvear, Diego; Wilson, Maxwell Z.
  • Rauch JN; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Valois E; Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, California, USA.
  • Solley SC; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Braig F; Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, California, USA.
  • Lach RS; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Audouard M; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Ponce-Rojas JC; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Costello MS; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Baxter NJ; Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, California, USA.
  • Kosik KS; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Arias C; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Acosta-Alvear D; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
  • Wilson MZ; University of California, Santa Barbara, Department of Molecular, Cellular, and Developmental Biology, Santa Barbara, California, USA.
J Clin Microbiol ; 59(4)2021 03 19.
Article in English | MEDLINE | ID: covidwho-1153633
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ABSTRACT
The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Jcm.02402-20

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Jcm.02402-20