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Perplexity vs Clarity in choosing the right molecular diagnostic techniques for SARS-COV2 detection in Indian setup.
Gogoi, Sanjib; Bora, Ishani; Debnath, Ekta; Sarkar, Subhabrata; Jais, Manoj B; Sharma, Amarjeet.
  • Gogoi S; Department of Microbiology, LHMC New Delhi, India.
  • Bora I; Department of Virology, PGIMER Chandigarh, India.
  • Debnath E; Department of Biochemistry LHMC New Delhi, India.
  • Sarkar S; Department of Virology, PGIMER Chandigarh, India.
  • Jais MB; Department of Microbiology, LHMC New Delhi, India.
  • Sharma A; Department of Virology, PGIMER Chandigarh, India.
J Family Med Prim Care ; 10(2): 615-624, 2021 Feb.
Article in English | MEDLINE | ID: covidwho-1154633
ABSTRACT
After the havoc created by Spanish flu a century ago, the world is witnessing exactly a similar pandemic situation since the beginning of the year 2020. The unexplained respiratory illness with high morbidity & mortality which started in Wuhan, China and spread across the world was finally termed as COVID-19 disease caused by SARS-CoV-2 and later announced as pandemic by WHO. This novel virus SARS-CoV-2 is a new variant of SARS corona virus with high infectivity and mysterious pathophysiology. The major step towards containment of this pandemic is to scale up the testing for SARS-CoV-2 and thereby isolating and managing the patients at the earliest. Molecular amplification based methods such a Real time Polymerase chain reaction (RT-PCR), CBNAAT and TrueNAT are the most commonly used techniques for detection of SARS-CoV2. To utilize these diagnostic facilities optimally in the management of the suspected COVID 19 patients, it is of utmost importance for the healthcare providers to understand the intricacies related to these technologies. Thus, the technical details along with the pros & cons of these three amplification-based technologies for proper understanding of these diagnostic modalities for SARS COV-2 diagnosis are discussed herewith.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: J Family Med Prim Care Year: 2021 Document Type: Article Affiliation country: Jfmpc.jfmpc_1793_20

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: J Family Med Prim Care Year: 2021 Document Type: Article Affiliation country: Jfmpc.jfmpc_1793_20