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Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium.
V'kovski, Philip; Gultom, Mitra; Kelly, Jenna N; Steiner, Silvio; Russeil, Julie; Mangeat, Bastien; Cora, Elisa; Pezoldt, Joern; Holwerda, Melle; Kratzel, Annika; Laloli, Laura; Wider, Manon; Portmann, Jasmine; Tran, Thao; Ebert, Nadine; Stalder, Hanspeter; Hartmann, Rune; Gardeux, Vincent; Alpern, Daniel; Deplancke, Bart; Thiel, Volker; Dijkman, Ronald.
  • V'kovski P; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Gultom M; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Kelly JN; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Steiner S; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Russeil J; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.
  • Mangeat B; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
  • Cora E; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Pezoldt J; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Holwerda M; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Kratzel A; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Laloli L; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.
  • Wider M; Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Portmann J; Gene Expression Core Facility (GECF), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Tran T; Gene Expression Core Facility (GECF), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Ebert N; Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Stalder H; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Hartmann R; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Gardeux V; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.
  • Alpern D; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
  • Deplancke B; Institute of Virology and Immunology (IVI), Bern, Switzerland.
  • Thiel V; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Dijkman R; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland.
PLoS Biol ; 19(3): e3001158, 2021 03.
Article in English | MEDLINE | ID: covidwho-1156073
Semantic information from SemMedBD (by NLM)
1. Epithelium LOCATION_OF SARS coronavirus
Subject
Epithelium
Predicate
LOCATION_OF
Object
SARS coronavirus
2. Epithelium LOCATION_OF 2019 novel coronavirus
Subject
Epithelium
Predicate
LOCATION_OF
Object
2019 novel coronavirus
3. Infected PROCESS_OF Persons
Subject
Infected
Predicate
PROCESS_OF
Object
Persons
4. Epithelium PART_OF Persons
Subject
Epithelium
Predicate
PART_OF
Object
Persons
5. Respiratory viruses COEXISTS_WITH 2019 novel coronavirus
Subject
Respiratory viruses
Predicate
COEXISTS_WITH
Object
2019 novel coronavirus
6. Epithelium LOCATION_OF SARS coronavirus
Subject
Epithelium
Predicate
LOCATION_OF
Object
SARS coronavirus
7. Epithelium LOCATION_OF 2019 novel coronavirus
Subject
Epithelium
Predicate
LOCATION_OF
Object
2019 novel coronavirus
8. Infected PROCESS_OF Persons
Subject
Infected
Predicate
PROCESS_OF
Object
Persons
9. Epithelium PART_OF Persons
Subject
Epithelium
Predicate
PART_OF
Object
Persons
10. Respiratory viruses COEXISTS_WITH 2019 novel coronavirus
Subject
Respiratory viruses
Predicate
COEXISTS_WITH
Object
2019 novel coronavirus
ABSTRACT
Since its emergence in December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread globally and become a major public health burden. Despite its close phylogenetic relationship to SARS-CoV, SARS-CoV-2 exhibits increased human-to-human transmission dynamics, likely due to efficient early replication in the upper respiratory epithelium of infected individuals. Since different temperatures encountered in the human upper and lower respiratory tract (33°C and 37°C, respectively) have been shown to affect the replication kinetics of several respiratory viruses, as well as host innate immune response dynamics, we investigated the impact of temperature on SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model. SARS-CoV-2, in contrast to SARS-CoV, replicated to higher titers when infections were performed at 33°C rather than 37°C. Although both viruses were highly sensitive to type I and type III interferon pretreatment, a detailed time-resolved transcriptome analysis revealed temperature-dependent interferon and pro-inflammatory responses induced by SARS-CoV-2 that were inversely proportional to its replication efficiency at 33°C or 37°C. These data provide crucial insight on pivotal virus-host interaction dynamics and are in line with characteristic clinical features of SARS-CoV-2 and SARS-CoV, as well as their respective transmission efficiencies.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Gene Expression Regulation, Viral / Gene Expression Profiling / SARS Virus / SARS-CoV-2 Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: PLoS Biol Journal subject: Biology Year: 2021 Document Type: Article Affiliation country: Journal.pbio.3001158

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Gene Expression Regulation, Viral / Gene Expression Profiling / SARS Virus / SARS-CoV-2 Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: PLoS Biol Journal subject: Biology Year: 2021 Document Type: Article Affiliation country: Journal.pbio.3001158