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A haemagglutination test for rapid detection of antibodies to SARS-CoV-2.
Townsend, Alain; Rijal, Pramila; Xiao, Julie; Tan, Tiong Kit; Huang, Kuan-Ying A; Schimanski, Lisa; Huo, Jiandong; Gupta, Nimesh; Rahikainen, Rolle; Matthews, Philippa C; Crook, Derrick; Hoosdally, Sarah; Dunachie, Susanna; Barnes, Eleanor; Street, Teresa; Conlon, Christopher P; Frater, John; Arancibia-Cárcamo, Carolina V; Rudkin, Justine; Stoesser, Nicole; Karpe, Fredrik; Neville, Matthew; Ploeg, Rutger; Oliveira, Marta; Roberts, David J; Lamikanra, Abigail A; Tsang, Hoi Pat; Bown, Abbie; Vipond, Richard; Mentzer, Alexander J; Knight, Julian C; Kwok, Andrew J; Screaton, Gavin R; Mongkolsapaya, Juthathip; Dejnirattisai, Wanwisa; Supasa, Piyada; Klenerman, Paul; Dold, Christina; Baillie, J Kenneth; Moore, Shona C; Openshaw, Peter J M; Semple, Malcolm G; Turtle, Lance C W; Ainsworth, Mark; Allcock, Alice; Beer, Sally; Bibi, Sagida; Skelly, Donal; Stafford, Lizzy; Jeffrey, Katie.
  • Townsend A; MRC Human Immunology Unit, MRC Weatherall Institute, John Radcliffe Hospital, Oxford, UK. alain.townsend@imm.ox.ac.uk.
  • Rijal P; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK. alain.townsend@imm.ox.ac.uk.
  • Xiao J; MRC Human Immunology Unit, MRC Weatherall Institute, John Radcliffe Hospital, Oxford, UK.
  • Tan TK; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Huang KA; MRC Human Immunology Unit, MRC Weatherall Institute, John Radcliffe Hospital, Oxford, UK.
  • Schimanski L; MRC Human Immunology Unit, MRC Weatherall Institute, John Radcliffe Hospital, Oxford, UK.
  • Huo J; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • Gupta N; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
  • Rahikainen R; MRC Human Immunology Unit, MRC Weatherall Institute, John Radcliffe Hospital, Oxford, UK.
  • Matthews PC; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Crook D; Structural Biology, The Rosalind Franklin Institute, Didcot, UK.
  • Hoosdally S; Vaccine Immunology Laboratory, National Institute of Immunology, New Delhi, India.
  • Dunachie S; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Barnes E; Department of Microbiology and Infectious Diseases, John Radcliffe Hospital, Oxford, UK.
  • Street T; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Conlon CP; Department of Microbiology and Infectious Diseases, John Radcliffe Hospital, Oxford, UK.
  • Frater J; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Arancibia-Cárcamo CV; Oxford NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford, UK.
  • Rudkin J; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Stoesser N; Oxford NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford, UK.
  • Karpe F; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Neville M; Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
  • Ploeg R; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Oliveira M; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Roberts DJ; Oxford NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford, UK.
  • Lamikanra AA; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Tsang HP; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Bown A; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Vipond R; Nuffield Department of Population Health, Big Data Institute, University of Oxford, Oxford, USA.
  • Mentzer AJ; Department of Microbiology and Infectious Diseases, John Radcliffe Hospital, Oxford, UK.
  • Knight JC; Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Kwok AJ; Oxford NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford, UK.
  • Screaton GR; Oxford Centre for Diabetes, Endocrinology and Metabolism, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Mongkolsapaya J; Oxford Centre for Diabetes, Endocrinology and Metabolism, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Dejnirattisai W; Nuffield Department of Surgical Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Supasa P; Nuffield Department of Surgical Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Klenerman P; NHS Blood and Transplant, John Radcliffe Hospital, Oxford, UK.
  • Dold C; BRC Haematology Theme and Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, USA.
  • Baillie JK; NHS Blood and Transplant, John Radcliffe Hospital, Oxford, UK.
  • Moore SC; NHS Blood and Transplant, John Radcliffe Hospital, Oxford, UK.
  • Openshaw PJM; Public Health England, Salisbury, UK.
  • Semple MG; Public Health England, Salisbury, UK.
  • Turtle LCW; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Ainsworth M; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Allcock A; Oxford NIHR Biomedical Research Centre, John Radcliffe Hospital, Oxford, UK.
  • Beer S; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Bibi S; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Skelly D; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Stafford L; Division of Medical Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Jeffrey K; Chinese Academy of Medical Science (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
Nat Commun ; 12(1): 1951, 2021 03 29.
Article in English | MEDLINE | ID: covidwho-1157905
ABSTRACT
Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Hemagglutination Tests / Spike Glycoprotein, Coronavirus / COVID-19 Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2021 Document Type: Article Affiliation country: S41467-021-22045-y

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Hemagglutination Tests / Spike Glycoprotein, Coronavirus / COVID-19 Testing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2021 Document Type: Article Affiliation country: S41467-021-22045-y