The relationship between serum levels of interleukin-2 and IL-8 with circulating microRNA-10b in patients with COVID-19.
Iran J Immunol
; 18(1): 65-73, 2021 03.
Article
in English
| MEDLINE | ID: covidwho-1159031
ABSTRACT
BACKGROUND:
The role of cytokine storm in the immunopathogenesis of coronavirus disease 2019 (COVID-19) has been implicated.OBJECTIVE:
To determine the association of microRNA (miRNA)-10b and serum levels of IL-2 and IL-8 in patients with COVID-19.METHODS:
Blood samples were obtained from 33 COVID-19 patients and 29 healthy subjects. After RNA extraction and cDNA synthesis, the transcript level of miR-10b was determined by Real-time PCR. In addition, the serum levels of IL-2 and IL-8 were measured in subjects using ELISA.RESULTS:
The patient group comprised of 33 patients with COVID-19 (62.4 ± 3.7 years old), 13 (39%) males and 20 (61%) females. In the control group, 29 subjects (56.6 ± 1.6 years old), 9 (31%) males and 20 (69%) females, were included. The expression of miR-10b was significantly downregulated in the peripheral blood of COVID-19 patients in comparison to the healthy controls (fold change= 0.12, P< 0.0001). The levels of IL-2 (P< 0.001) and IL-8 (P< 0.001) were significantly increased in the serum samples of COVID-19 patients compared to the healthy subjects. The expression level of miR-10b was correlated significantly with the serum levels of IL-2 and IL-8 as well as with the age of patients, ESR and CRP levels.CONCLUSIONS:
miR-10b is downregulated in the COVID-19 patients and might result in increased levels of IL-2 and IL-8, hence contributing to cytokine storm.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Interleukin-8
/
Interleukin-2
/
MicroRNAs
/
Circulating MicroRNA
/
SARS-CoV-2
/
COVID-19
Type of study:
Experimental Studies
/
Observational study
/
Prognostic study
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
English
Journal:
Iran J Immunol
Year:
2021
Document Type:
Article
Affiliation country:
Iji.2021.88780.1904
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