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Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitors.
Kiew, Lik-Voon; Chang, Chia-Yu; Huang, Sheng-Yu; Wang, Pei-Wen; Heh, Choon-Han; Liu, Chung-Te; Cheng, Chia-Hsin; Lu, Yi-Xiang; Chen, Yen-Chen; Huang, Yi-Xuan; Chang, Sheng-Yun; Tsai, Huei-Yu; Kung, Yu-An; Huang, Peng-Nien; Hsu, Ming-Hua; Leo, Bey-Fen; Foo, Yiing-Yee; Su, Chien-Hao; Hsu, Kuo-Chen; Huang, Po-Hsun; Ng, Chirk-Jenn; Kamarulzaman, Adeeba; Yuan, Chiun-Jye; Shieh, Dar-Bin; Shih, Shin-Ru; Chung, Lip-Yong; Chang, Chia-Ching.
  • Kiew LV; Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Chang CY; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Huang SY; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memo
  • Wang PW; Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan.
  • Heh CH; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Liu CT; Division of Nephrology, Department of Internal Medicine, Wan-Fang Hospital, Taipei Medical University, Taipei, 11696, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan.
  • Cheng CH; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Lu YX; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Chen YC; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Huang YX; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Chang SY; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memo
  • Tsai HY; Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan.
  • Kung YA; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memo
  • Huang PN; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memo
  • Hsu MH; Department of Chemistry, National Changhua University of Education, Changhua, 50007, Taiwan.
  • Leo BF; Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Foo YY; Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Su CH; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Hsu KC; Pd Biomedical Co., Ltd., Taoyuan, 32560, Taiwan.
  • Huang PH; Institute of Clinical Medicine, Cardiovascular Research Center, National Yang Ming Chiao Tung University, Taipei, 11121, Taiwan; Division of Cardiology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan.
  • Ng CJ; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Kamarulzaman A; Department of Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Yuan CJ; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan.
  • Shieh DB; Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan; Center for Micro/Nano Science and Tech
  • Shih SR; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memo
  • Chung LY; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: chungly@um.edu.my.
  • Chang CC; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Depa
Biosens Bioelectron ; 183: 113213, 2021 Jul 01.
Article in English | MEDLINE | ID: covidwho-1163433
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of its spike protein (S-protein) to the cell surface-expressing angiotensin-converting enzyme 2 (ACE2). Thus, inhibition of S-protein-ACE2 binding may impede SARS-CoV-2 cell entry and attenuate the progression of Coronavirus disease 2019 (COVID-19). In this study, an electrochemical impedance spectroscopy-based biosensing platform consisting of a recombinant ACE2-coated palladium nano-thin-film electrode as the core sensing element was fabricated for the screening of potential inhibitors against S-protein-ACE2 binding. The platform could detect interference of small analytes against S-protein-ACE2 binding at low analyte concentration and small volume (0.1 µg/mL and ~1 µL, estimated total analyte consumption < 4 pg) within 21 min. Thus, a few potential inhibitors of S-protein-ACE2 binding were identified. This includes (2S,3aS,6aS)-1-((S)-N-((S)-1-Carboxy-3-phenylpropyl)alanyl)tetrahydrocyclopenta[b] pyrrole-2-carboxylic acid (ramiprilat) and (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-Carboxybutyl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid (perindoprilat) that reduced the binding affinity of S-protein to ACE2 by 72% and 67%; and SARS-CoV-2 in vitro infectivity to the ACE2-expressing human oral cavity squamous carcinoma cells (OEC-M1) by 36.4 and 20.1%, respectively, compared to the PBS control. These findings demonstrated the usefulness of the developed biosensing platform for the rapid screening of modulators for S-protein-ACE2 binding.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113213

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113213