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A Highly Automated Mobile Laboratory for On-site Molecular Diagnostics in the COVID-19 Pandemic.
Xing, Wanli; Wang, Jiadao; Zhao, Chao; Wang, Han; Bai, Liang; Pan, Liangbin; Li, Hang; Wang, Huili; Zhang, Zhi; Lu, Ying; Chen, Xiang; Shan, Sisi; Wang, Dong; Pan, Yifei; Weng, Ding; Zhou, Xinying; Huang, Rudan; He, Jianxing; Jin, Ronghua; Li, Weimin; Shang, Hong; Zhong, Nanshan; Cheng, Jing.
  • Xing W; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
  • Wang J; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Zhao C; CapitalBio Technology, Beijing, China.
  • Wang H; State Key Laboratory of Tribology, Tsinghua University, Beijing, China.
  • Bai L; Department of Industrial Design, Academy of Arts & Design, Tsinghua University, Beijing, China.
  • Pan L; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
  • Li H; CapitalBio Technology, Beijing, China.
  • Wang H; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Zhang Z; CapitalBio Technology, Beijing, China.
  • Lu Y; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Chen X; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
  • Shan S; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Wang D; Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
  • Pan Y; CapitalBio Technology, Beijing, China.
  • Weng D; Center for Global Health and Infectious Diseases, Comprehensive AIDS Research Center, Beijing, Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, Beijing, China.
  • Zhou X; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Huang R; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • He J; State Key Laboratory of Tribology, Tsinghua University, Beijing, China.
  • Jin R; CapitalBio Technology, Beijing, China.
  • Li W; National Engineering Research Center for Beijing Biochip Technology, Beijing, China.
  • Shang H; State Key Laboratory of Respiratory Disease and National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
  • Zhong N; Beijing Ditan Hospital, Capital Medical University, Beijing, China.
  • Cheng J; Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China.
Clin Chem ; 67(4): 672-683, 2021 03 31.
Article in English | MEDLINE | ID: covidwho-1165392
Semantic information from SemMedBD (by NLM)
1. Diagnosis DIAGNOSES COVID-19
Subject
Diagnosis
Predicate
DIAGNOSES
Object
COVID-19
2. Diagnosis DIAGNOSES COVID-19
Subject
Diagnosis
Predicate
DIAGNOSES
Object
COVID-19
ABSTRACT

BACKGROUND:

Infectious disease outbreaks such as the COVID-19 (coronavirus disease 2019) pandemic call for rapid response and complete screening of the suspected community population to identify potential carriers of pathogens. Central laboratories rely on time-consuming sample collection methods that are rarely available in resource-limited settings.

METHODS:

We present a highly automated and fully integrated mobile laboratory for fast deployment in response to infectious disease outbreaks. The mobile laboratory was equipped with a 6-axis robot arm for automated oropharyngeal swab specimen collection; virus in the collected specimen was inactivated rapidly using an infrared heating module. Nucleic acid extraction and nested isothermal amplification were performed by a "sample in, answer out" laboratory-on-a-chip system, and the result was automatically reported by the onboard information platform. Each module was evaluated using pseudovirus or clinical samples.

RESULTS:

The mobile laboratory was stand-alone and self-sustaining and capable of on-site specimen collection, inactivation, analysis, and reporting. The automated sampling robot arm achieved sampling efficiency comparable to manual collection. The collected samples were inactivated in as short as 12 min with efficiency comparable to a water bath without damage to nucleic acid integrity. The limit of detection of the integrated microfluidic nucleic acid analyzer reached 150 copies/mL within 45 min. Clinical evaluation of the onboard microfluidic nucleic acid analyzer demonstrated good consistency with reverse transcription quantitative PCR with a κ coefficient of 0.979.

CONCLUSIONS:

The mobile laboratory provides a promising solution for fast deployment of medical diagnostic resources at critical junctions of infectious disease outbreaks and facilitates local containment of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) transmission.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Pathology, Molecular / COVID-19 Nucleic Acid Testing / COVID-19 / Laboratories / Mobile Health Units Type of study: Diagnostic study / Clinical Practice Guide / Prognostic study Limits: Adult / Female / Humans / Male Language: English Journal: Clin Chem Journal subject: Chemistry, Clinical Year: 2021 Document Type: Article Affiliation country: Clinchem

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Pathology, Molecular / COVID-19 Nucleic Acid Testing / COVID-19 / Laboratories / Mobile Health Units Type of study: Diagnostic study / Clinical Practice Guide / Prognostic study Limits: Adult / Female / Humans / Male Language: English Journal: Clin Chem Journal subject: Chemistry, Clinical Year: 2021 Document Type: Article Affiliation country: Clinchem