ATeam technology for detecting early signs of viral cytopathic effect.
J Vet Med Sci
; 82(3): 387-393, 2020 Mar 24.
Article
in English
| MEDLINE | ID: covidwho-11655
ABSTRACT
Adenosine 5'-triphosphate (ATP), the major energy currency of the cell, is involved in many cellular processes, including the viral life cycle, and can be used as an indicator of early signs of cytopathic effect (CPE). In this study, we demonstrated that CPE can be analyzed using an FRET-based ATP probe named ATP indicator based on Epsilon subunit for Analytical Measurements (ATeam). The results revealed that as early as 3 hr, the virus infected cells showed a significantly different Venus/cyan fluorescent protein (CFP) ratio compared to the mock-infected cells. The ATeam technology is therefore useful to determine the early signs of ATP-based CPE as early as 3 hr without morphology-based CPE by light microscopy, and enables high throughput determination of the presence of microorganisms in neglected samples stored in laboratories.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viruses
/
Adenosine Triphosphate
/
Cytopathogenic Effect, Viral
/
Fluorescence Resonance Energy Transfer
Type of study:
Experimental Studies
Limits:
Animals
/
Humans
Language:
English
Journal:
J Vet Med Sci
Journal subject:
Veterinary Medicine
Year:
2020
Document Type:
Article
Affiliation country:
Jvms.20-0021
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