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ATeam technology for detecting early signs of viral cytopathic effect.
Doysabas, Karla Cristine C; Oba, Mami; Ishibashi, Tomoki; Shibata, Hideki; Takemae, Hitoshi; Shimoda, Hiroshi; Tarigan, Ronald; Mizutani, Tetsuya; Iida, Atsuo; Hondo, Eiichi.
  • Doysabas KCC; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Oba M; Laboratory of Veterinary Microbiology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Sawai, Fuchu, Tokyo 183-8509, Japan.
  • Ishibashi T; Laboratory of Insect Ecology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8224, Japan.
  • Shibata H; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Takemae H; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Shimoda H; Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8511, Japan.
  • Tarigan R; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Mizutani T; Laboratory of Veterinary Microbiology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Sawai, Fuchu, Tokyo 183-8509, Japan.
  • Iida A; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
  • Hondo E; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
J Vet Med Sci ; 82(3): 387-393, 2020 Mar 24.
Article in English | MEDLINE | ID: covidwho-11655
ABSTRACT
Adenosine 5'-triphosphate (ATP), the major energy currency of the cell, is involved in many cellular processes, including the viral life cycle, and can be used as an indicator of early signs of cytopathic effect (CPE). In this study, we demonstrated that CPE can be analyzed using an FRET-based ATP probe named ATP indicator based on Epsilon subunit for Analytical Measurements (ATeam). The results revealed that as early as 3 hr, the virus infected cells showed a significantly different Venus/cyan fluorescent protein (CFP) ratio compared to the mock-infected cells. The ATeam technology is therefore useful to determine the early signs of ATP-based CPE as early as 3 hr without morphology-based CPE by light microscopy, and enables high throughput determination of the presence of microorganisms in neglected samples stored in laboratories.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses / Adenosine Triphosphate / Cytopathogenic Effect, Viral / Fluorescence Resonance Energy Transfer Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: J Vet Med Sci Journal subject: Veterinary Medicine Year: 2020 Document Type: Article Affiliation country: Jvms.20-0021

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses / Adenosine Triphosphate / Cytopathogenic Effect, Viral / Fluorescence Resonance Energy Transfer Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: J Vet Med Sci Journal subject: Veterinary Medicine Year: 2020 Document Type: Article Affiliation country: Jvms.20-0021