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Development and Validation of a Multiplex Microsphere Immunoassay Using Dried Blood Spots for SARS-CoV-2 Seroprevalence: Application in First Responders in Colorado, USA.
Schultz, Jonathan S; McCarthy, Mary K; Rester, Cody; Sabourin, Katherine R; Annen, Kyle; DomBourian, Melkon; Eisenmesser, Elan; Frazer-Abel, Ashley; Knight, Vijaya; Jaenisch, Thomas; Morrison, Thomas E; Rochford, Rosemary; Kedl, Ross M.
  • Schultz JS; Division of Infectious Diseases, School of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • McCarthy MK; Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Rester C; Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Sabourin KR; Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Annen K; Clinical & Translational Allergy and Immunology Laboratory, Children's Hospital of Colorado, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • DomBourian M; Clinical & Translational Allergy and Immunology Laboratory, Children's Hospital of Colorado, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Eisenmesser E; Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Frazer-Abel A; Exsera Biolabs, Department of Rheumatology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Knight V; Clinical & Translational Allergy and Immunology Laboratory, Children's Hospital of Colorado, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Jaenisch T; Department of Epidemiology, Colorado School of Public Health, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Morrison TE; Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA.
  • Rochford R; Department of Immunology and Microbiology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USA Rosemary.Rochford@cuanschutz.edu.
  • Kedl RM; Department of Epidemiology, Colorado School of Public Health, Anschutz Medical Campus, Aurora, Colorado, USA.
J Clin Microbiol ; 59(6)2021 05 19.
Article in English | MEDLINE | ID: covidwho-1255519
ABSTRACT
Serological testing of large representative populations for antibodies to SARS-CoV-2 is needed to estimate seroprevalence, transmission dynamics, and the duration of antibody responses from natural infection and vaccination. In this study, a high-throughput SARS-CoV-2 multiplex microsphere immunoassay (MMIA) was developed for the receptor binding domain (RBD) and nucleocapsid (N) that was more sensitive than enzyme-linked immunosorbent assay (ELISA) (98% versus 87%). The MMIA was then applied and validated in 264 first responders in Colorado using serum and dried blood spot (DBS) eluates, compared to ELISA, and evaluated for neutralizing antibodies. Four percent (11/264) of first responders were seropositive in July to August 2020. Serum and DBS were highly correlated for anti-RBD and anti-N antibodies (R = 0.83, P < 0.0001 and R = 0.87, P < 0.0001, respectively) by MMIA. The MMIA accurately predicted SARS-CoV-2 neutralizing antibodies using DBS (R = 0.76, P = 0.037). On repeat antibody testing 3 months later, anti-RBD IgG decreased less rapidly than anti-N IgG measured by MMIA, with a median change in geometric median fluorescence intensity of 62% versus 79% (P < 0.01) for anti-RBD and anti-N IgG, respectively. This novel MMIA using DBS could be scalable for rapid and affordable SARS-CoV-2 serosurveillance in the United States and globally.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Emergency Responders / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: North America Language: English Year: 2021 Document Type: Article Affiliation country: Jcm.00290-21

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Emergency Responders / COVID-19 Type of study: Experimental Studies / Observational study / Prognostic study Topics: Vaccines Limits: Humans Country/Region as subject: North America Language: English Year: 2021 Document Type: Article Affiliation country: Jcm.00290-21