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RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2.
Huang, Wei E; Lim, Boon; Hsu, Chia-Chen; Xiong, Dan; Wu, Wei; Yu, Yejiong; Jia, Huidong; Wang, Yun; Zeng, Yida; Ji, Mengmeng; Chang, Hong; Zhang, Xiuming; Wang, Hui; Cui, Zhanfeng.
  • Huang WE; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
  • Lim B; Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, UK.
  • Hsu CC; Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, UK.
  • Xiong D; Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, UK.
  • Wu W; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, OX3 7DQ, UK.
  • Yu Y; Medical Laboratory of Shenzhen Luohu People's Hospital, Shenzhen, 518001, China.
  • Jia H; Medical Laboratory of Shenzhen Luohu People's Hospital, Shenzhen, 518001, China.
  • Wang Y; Department of Engineering Science, University of Oxford, Parks Road, OX1 3PJ, Oxford, UK.
  • Zeng Y; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, OX3 7DQ, UK.
  • Ji M; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
  • Chang H; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
  • Zhang X; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
  • Wang H; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
  • Cui Z; Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou Industrial Park, Jiangsu, China.
Microb Biotechnol ; 13(4): 950-961, 2020 07.
Article in English | MEDLINE | ID: covidwho-116666
Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus PROCESS_OF World
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
World
2. Oligonucleotide Primers INTERACTS_WITH RN
Subject
Oligonucleotide Primers
Predicate
INTERACTS_WITH
Object
RN
3. 2019 novel coronavirus PROCESS_OF World
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
World
4. Oligonucleotide Primers INTERACTS_WITH RNA, Viral
Subject
Oligonucleotide Primers
Predicate
INTERACTS_WITH
Object
RNA, Viral
ABSTRACT
The pandemic coronavirus SARS-CoV-2 in the world has caused a large infected population suffering from COVID-19. To curb the spreading of the virus, WHO urgently demanded an extension of screening and testing; thus, a rapid and simple diagnostic method is needed. We applied a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) to achieve the detection of SARS-CoV-2 in 30 min. We designed four sets of LAMP primers (6 primers in each set), targeting the viral RNA of SARS-CoV-2 in the regions of orf1ab, S gene and N gene. A colorimetric change was used to report the results, which enables the outcome of viral RNA amplification to be read by the naked eye without the need of expensive or dedicated instrument. The sensitivity can be 80 copies of viral RNA per ml in a sample. We validated the RT-LAMP method in a hospital in China, employing 16 clinic samples with 8 positives and 8 negatives. The testing results are consistent with the conventional RT-qPCR. In addition, we also show that one-step process without RNA extraction is feasible to achieve RNA amplification directly from a sample. This rapid, simple and sensitive RT-LAMP method paves a way for a large screening at public domain and hospitals, particularly regional hospitals and medical centres in rural areas.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Type of study: Diagnostic study / Evaluation study Language: English Journal: Microb Biotechnol Clinical aspect: Diagnosis Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Nucleic Acid Amplification Techniques / Betacoronavirus Type of study: Diagnostic study / Evaluation study Language: English Journal: Microb Biotechnol Clinical aspect: Diagnosis Year: 2020
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