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Absence of SARS-CoV-2 (COVID-19 virus) within the IVF laboratory using strict patient screening and safety criteria.
Rajput, Sandeep K; Khan, Shaihla A; Goheen, Benjamin B; Engelhorn, Heidi J; Logsdon, Deirdre M; Grimm, Courtney K; Kile, Rebecca A; West, Rachel C; Yuan, Ye; Schoolcraft, William B; McCormick, Sue; Krisher, Rebecca L; Swain, Jason E.
  • Rajput SK; CCRM Colorado, Lone Tree CO, USA.
  • Khan SA; CCRM Colorado, Lone Tree CO, USA.
  • Goheen BB; CCRM Colorado, Lone Tree CO, USA.
  • Engelhorn HJ; CCRM Colorado, Lone Tree CO, USA.
  • Logsdon DM; CCRM Colorado, Lone Tree CO, USA.
  • Grimm CK; CCRM Colorado, Lone Tree CO, USA.
  • Kile RA; CCRM Colorado, Lone Tree CO, USA.
  • West RC; CCRM Colorado, Lone Tree CO, USA.
  • Yuan Y; CCRM Colorado, Lone Tree CO, USA.
  • Schoolcraft WB; CCRM Colorado, Lone Tree CO, USA.
  • McCormick S; CCRM Colorado, Lone Tree CO, USA.
  • Krisher RL; CCRM Colorado, Lone Tree CO, USA.
  • Swain JE; CCRM Fertility Network, Lone Tree CO, USA. Electronic address: jswain@ccrmivf.com.
Reprod Biomed Online ; 42(6): 1067-1074, 2021 06.
Article in English | MEDLINE | ID: covidwho-1169278
ABSTRACT
RESEARCH QUESTION Is there a risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral exposure and potential cross-contamination from follicular fluid, culture media and vitrification solution within the IVF laboratory using strict patient screening and safety measures?

DESIGN:

This was a prospective clinical study. All women undergoing transvaginal oocyte retrieval were required to have a negative SARS-CoV-2 RNA test 3-5 days prior to the procedure. Male partners were not tested. All cases used intracytoplasmic sperm injection (ICSI). The first tube of follicular fluid aspirated during oocyte retrieval, drops of media following removal of the embryos on day 5, and vitrification solution after blastocyst cryopreservation were analysed for SARS-CoV-2 RNA.

RESULTS:

In total, medium from 61 patients, vitrification solution from 200 patients and follicular fluid from 300 patients was analysed. All samples were negative for SARS-CoV-2 viral RNA.

CONCLUSIONS:

With stringent safety protocols in place, including testing of women and symptom-based screening of men, the presence of SARS-CoV-2 RNA was not detected in follicular fluid, medium or vitrification solution. This work demonstrates the possibility of implementing a rapid laboratory screening assay for SARS-CoV-2 and has implications for safe laboratory operations, including cryostorage recommendations.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Fertilization in Vitro / Follicular Fluid / Culture Media / SARS-CoV-2 / Laboratories Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study / Randomized controlled trials Limits: Female / Humans Language: English Journal: Reprod Biomed Online Journal subject: Reproductive Medicine Year: 2021 Document Type: Article Affiliation country: J.rbmo.2021.03.005

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Fertilization in Vitro / Follicular Fluid / Culture Media / SARS-CoV-2 / Laboratories Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study / Randomized controlled trials Limits: Female / Humans Language: English Journal: Reprod Biomed Online Journal subject: Reproductive Medicine Year: 2021 Document Type: Article Affiliation country: J.rbmo.2021.03.005