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Circulating Nucleosomes as Potential Markers to Monitor COVID-19 Disease Progression.
Cavalier, Etienne; Guiot, Julien; Lechner, Katharina; Dutsch, Alexander; Eccleston, Mark; Herzog, Marielle; Bygott, Thomas; Schomburg, Adrian; Kelly, Theresa; Holdenrieder, Stefan.
  • Cavalier E; Clinical Chemistry Department, Centre Hospitalier Universitaire de Liege, Domaine Universitaire Du Sart-Tilman, Liège, Belgium.
  • Guiot J; Pneumology Department, Centre Hospitalier Universitaire de Liege, Domaine Universitaire Du Sart-Tilman, Liège, Belgium.
  • Lechner K; Department of Cardiology, German Heart Centre Munich, Technical University Munich, Munich, Germany.
  • Dutsch A; DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance, Munich, Germany.
  • Eccleston M; Department of Cardiology, German Heart Centre Munich, Technical University Munich, Munich, Germany.
  • Herzog M; DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance, Munich, Germany.
  • Bygott T; Belgian Volition SRL, Isnes, Belgium.
  • Schomburg A; Belgian Volition SRL, Isnes, Belgium.
  • Kelly T; Belgian Volition SRL, Isnes, Belgium.
  • Holdenrieder S; Eisbach Bio GmbH, Munich, Germany.
Front Mol Biosci ; 8: 600881, 2021.
Article in English | MEDLINE | ID: covidwho-1170100
ABSTRACT
The severity of coronavirus disease 2019 (COVID-19) varies significantly with cases spanning from asymptomatic to lethal with a subset of individuals developing Severe Acute Respiratory Syndrome (SARS) and death from respiratory failure. To determine whether global nucleosome and citrullinated nucleosome levels were elevated in COVID-19 patients, we tested two independent cohorts of COVID-19 positive patients with quantitative nucleosome immunoassays and found that nucleosomes were highly elevated in plasma of COVID-19 patients with a severe course of the disease relative to healthy controls and that both histone 3.1 variant and citrullinated nucleosomes increase with disease severity. Elevated citrullination of circulating nucleosomes is indicative of neutrophil extracellular trap formation, neutrophil activation and NETosis in severely affected individuals. Importantly, using hospital setting (outpatient, inpatient or ICU) as a proxy for disease severity, nucleosome levels increased with disease severity and may serve as a guiding biomarker for treatment. Owing to the limited availability of mechanical ventilators and extracorporal membrane oxygenation (ECMO) equipment, there is an urgent need for effective tools to rapidly assess disease severity and guide treatment selection. Based on our studies of two independent cohorts of COVID-19 patients from Belgium and Germany, we suggest further investigation of circulating nucleosomes and citrullination as biomarkers for clinical triage, treatment allocation and clinical drug discovery.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Observational study / Prognostic study Topics: Variants Language: English Journal: Front Mol Biosci Year: 2021 Document Type: Article Affiliation country: Fmolb.2021.600881

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Cohort study / Observational study / Prognostic study Topics: Variants Language: English Journal: Front Mol Biosci Year: 2021 Document Type: Article Affiliation country: Fmolb.2021.600881