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Aligner-Mediated Cleavage-Based Isothermal Amplification for SARS-CoV-2 RNA Detection.
Zhang, Chao; Zheng, Tingting; Fan, Hongliang; Zhang, Tao; Han, Da.
  • Zhang C; Institute of Molecular Medicine (IMM), Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
  • Zheng T; Institute of Molecular Medicine (IMM), Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
  • Fan H; Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China.
  • Zhang T; Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China.
  • Han D; Institute of Molecular Medicine (IMM), Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
ACS Appl Bio Mater ; 4(5): 3805-3810, 2021 05 17.
Article in English | MEDLINE | ID: covidwho-1172543
ABSTRACT
Rapid detection of SARS-CoV-2 RNA is critical for reducing the global transmission of COVID-19. Here, we report a simple and versatile assay for detection of SARS-CoV-2 RNA based on aligner-mediated cleavage-based strand displacement amplification (AMC-SDA). The entire amplification procedure takes less than 25 min without professional instruments or requirement of specific target sequences and can reach a limit of detection of attomolar RNA concentration. Using pseudovirus as mimicry of clinical SARS-CoV-2 positive samples, we achieved a diagnostic accuracy of 100% in 10 simulated samples (five positive and five negative). We anticipate that our method will provide a universal platform for rapid and accurate detection of emerging infectious diseases.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Nucleic Acid Amplification Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Appl Bio Mater Year: 2021 Document Type: Article Affiliation country: Acsabm.0c01674

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Nucleic Acid Amplification Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: ACS Appl Bio Mater Year: 2021 Document Type: Article Affiliation country: Acsabm.0c01674