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Evaluation of 11 SARS-CoV-2 antibody tests by using samples from patients with defined IgG antibody titers.
Lagerqvist, Nina; Maleki, Kimia T; Verner-Carlsson, Jenny; Olausson, Mikaela; Dillner, Joakim; Wigren Byström, Julia; Monsen, Tor; Forsell, Mattias; Eriksson, Jenny; Bogdanovic, Gordana; Muschiol, Sandra; Ljunggren, Joel; Repo, Johanna; Kjerstadius, Torbjörn; Muradrasoli, Shaman; Brytting, Mia; Szekely Björndal, Åsa; Åkerlund, Thomas; Nilsson, Charlotta; Klingström, Jonas.
  • Lagerqvist N; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden. nina.lagerqvist@folkhalsomyndigheten.se.
  • Maleki KT; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Verner-Carlsson J; Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
  • Olausson M; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Dillner J; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Wigren Byström J; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Monsen T; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
  • Forsell M; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
  • Eriksson J; Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
  • Bogdanovic G; Region Västmanland, Västerås, Sweden.
  • Muschiol S; Karolinska University Hospital, Stockholm, Sweden.
  • Ljunggren J; Karolinska University Hospital, Stockholm, Sweden.
  • Repo J; Region Västernorrland, County Hospital of Västernorrland, Sundsvall, Sweden.
  • Kjerstadius T; Region Västernorrland, County Hospital of Västernorrland, Sundsvall, Sweden.
  • Muradrasoli S; Region Värmland, Centralsjukhuset, Karlstad, Sweden.
  • Brytting M; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Szekely Björndal Å; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Åkerlund T; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Nilsson C; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
  • Klingström J; Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
Sci Rep ; 11(1): 7614, 2021 04 07.
Article in English | MEDLINE | ID: covidwho-1172565
ABSTRACT
We evaluated the performance of 11 SARS-CoV-2 antibody tests using a reference set of heat-inactivated samples from 278 unexposed persons and 258 COVID-19 patients, some of whom contributed serial samples. The reference set included samples with a variation in SARS-CoV-2 IgG antibody titers, as determined by an in-house immunofluorescence assay (IFA). The five evaluated rapid diagnostic tests had a specificity of 99.0% and a sensitivity that ranged from 56.3 to 81.6% and decreased with low IFA IgG titers. The specificity was > 99% for five out of six platform-based tests, and when assessed using samples collected ≥ 22 days after symptom onset, two assays had a sensitivity of > 96%. These two assays also detected samples with low IFA titers more frequently than the other assays. In conclusion, the evaluated antibody tests showed a heterogeneity in their performances and only a few tests performed well with samples having low IFA IgG titers, an important aspect for diagnostics and epidemiological investigations.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-87289-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Limits: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-87289-6