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Intranasal versus intratracheal exposure to lipopolysaccharides in a murine model of acute respiratory distress syndrome.
Khadangi, Fatemeh; Forgues, Anne-Sophie; Tremblay-Pitre, Sophie; Dufour-Mailhot, Alexis; Henry, Cyndi; Boucher, Magali; Beaulieu, Marie-Josée; Morissette, Mathieu; Fereydoonzad, Liah; Brunet, David; Robichaud, Annette; Bossé, Ynuk.
  • Khadangi F; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Forgues AS; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Tremblay-Pitre S; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Dufour-Mailhot A; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Henry C; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Boucher M; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Beaulieu MJ; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Morissette M; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada.
  • Fereydoonzad L; SCIREQ - Scientific Respiratory Equipment Inc., Montreal, Canada.
  • Brunet D; SCIREQ - Scientific Respiratory Equipment Inc., Montreal, Canada.
  • Robichaud A; SCIREQ - Scientific Respiratory Equipment Inc., Montreal, Canada.
  • Bossé Y; Institut Universitaire de Cardiologie et de Pneumologie de Québec - Université Laval, Pavillon Mallet, M2694, 2725, chemin Sainte-Foy, Quebec, QC, G1V 4G5, Canada. ynuk.bosse@criucpq.ulaval.ca.
Sci Rep ; 11(1): 7777, 2021 04 08.
Article in English | MEDLINE | ID: covidwho-1174704
Semantic information from SemMedBD (by NLM)
1. Lung LOCATION_OF Mildly Abnormal Ejection Fraction
Subject
Lung
Predicate
LOCATION_OF
Object
Mildly Abnormal Ejection Fraction
2. Lipopolysaccharides CAUSES Respiratory Distress Syndrom
Subject
Lipopolysaccharides
Predicate
CAUSES
Object
Respiratory Distress Syndrom
3. Interventional procedure AFFECTS Complication
Subject
Interventional procedure
Predicate
AFFECTS
Object
Complication
4. Lung LOCATION_OF Mildly Abnormal Ejection Fraction
Subject
Lung
Predicate
LOCATION_OF
Object
Mildly Abnormal Ejection Fraction
5. Lipopolysaccharides CAUSES Respiratory Distress Syndrome, Adult
Subject
Lipopolysaccharides
Predicate
CAUSES
Object
Respiratory Distress Syndrome, Adult
6. Interventional procedure AFFECTS Complication
Subject
Interventional procedure
Predicate
AFFECTS
Object
Complication
ABSTRACT
Due to frequent and often severe lung affections caused by COVID-19, murine models of acute respiratory distress syndrome (ARDS) are increasingly used in experimental lung research. The one induced by a single lipopolysaccharide (LPS) exposure is practical. However, whether it is preferable to administer LPS intranasally or intratracheally remains an open question. Herein, female C57Bl/6 J mice were exposed intranasally or intratracheally to one dose of either saline or 3 mg/kg of LPS. They were studied 24 h later. The groups treated with LPS, either intranasally or intratracheally, exhibited a pronounced neutrophilic inflammation, signs of lung tissue damage and protein extravasation into the alveoli, and mild lung dysfunction. The magnitude of the response was generally not different between groups exposed intranasally versus intratracheally. However, the variability of some the responses was smaller in the LPS-treated groups exposed intranasally versus intratracheally. Notably, the saline-treated mice exposed intratracheally demonstrated a mild neutrophilic inflammation and alterations of the airway epithelium. We conclude that an intranasal exposure is as effective as an intratracheal exposure in a murine model of ARDS induced by LPS. Additionally, the groups exposed intranasally demonstrated less variability in the responses to LPS and less complications associated with the sham procedure.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Distress Syndrome / Lipopolysaccharides / Inflammation / Lung Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-87462-x

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Respiratory Distress Syndrome / Lipopolysaccharides / Inflammation / Lung Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-87462-x