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Role of Immunoglobulin M and A Antibodies in the Neutralization of Severe Acute Respiratory Syndrome Coronavirus 2.
Klingler, Jéromine; Weiss, Svenja; Itri, Vincenza; Liu, Xiaomei; Oguntuyo, Kasopefoluwa Y; Stevens, Christian; Ikegame, Satoshi; Hung, Chuan-Tien; Enyindah-Asonye, Gospel; Amanat, Fatima; Baine, Ian; Arinsburg, Suzanne; Bandres, Juan C; Kojic, Erna Milunka; Stoever, Jonathan; Jurczyszak, Denise; Bermudez-Gonzalez, Maria; Nádas, Arthur; Liu, Sean; Lee, Benhur; Zolla-Pazner, Susan; Hioe, Catarina E.
  • Klingler J; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Weiss S; James J. Peters VA Medical Center, Bronx, New York, USA.
  • Itri V; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Liu X; James J. Peters VA Medical Center, Bronx, New York, USA.
  • Oguntuyo KY; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Stevens C; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Ikegame S; James J. Peters VA Medical Center, Bronx, New York, USA.
  • Hung CT; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Enyindah-Asonye G; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Amanat F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Baine I; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Arinsburg S; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Bandres JC; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Kojic EM; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Stoever J; Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Jurczyszak D; Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Bermudez-Gonzalez M; James J. Peters VA Medical Center, Bronx, New York, USA.
  • Nádas A; Division of Infectious Diseases, Department of Medicine, Mount Sinai West and Morningside, New York, USA.
  • Liu S; Pulmonary and Critical Care Medicine, Mount Sinai West, New York, USA.
  • Lee B; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Zolla-Pazner S; Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
  • Hioe CE; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
J Infect Dis ; 223(6): 957-970, 2021 03 29.
Article in English | MEDLINE | ID: covidwho-1174906
ABSTRACT

BACKGROUND:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people globally. Virus infection requires the receptor-binding domain (RBD) of the spike protein. Although studies have demonstrated anti-spike and -RBD antibodies to be protective in animal models, and convalescent plasma as a promising therapeutic option, little is known about immunoglobulin isotypes capable of blocking infection.

METHODS:

We studied spike- and RBD-specific immunoglobulin isotypes in convalescent and acute plasma/serum samples using a multiplex bead assay. We also determined virus neutralization activities in plasma and serum samples, and purified immunoglobulin fractions using a vesicular stomatitis pseudovirus assay.

RESULTS:

Spike- and RBD-specific immunoglobulin (Ig) M, IgG1, and IgA1 were produced by all or nearly all subjects at variable levels and detected early after infection. All samples displayed neutralizing activity. Regression analyses revealed that IgM and IgG1 contributed most to neutralization, consistent with IgM and IgG fractions' neutralization potency. IgA also exhibited neutralizing activity, but with lower potency.

CONCLUSION:

IgG, IgM, and IgA are critical components of convalescent plasma used for treatment of coronavirus disease 2019 (COVID-19).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin A / Immunoglobulin M / Antibodies, Neutralizing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: J Infect Dis Year: 2021 Document Type: Article Affiliation country: Infdis

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin A / Immunoglobulin M / Antibodies, Neutralizing / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Limits: Female / Humans / Male Language: English Journal: J Infect Dis Year: 2021 Document Type: Article Affiliation country: Infdis