Your browser doesn't support javascript.
Development of a Colorimetric Tool for SARS-CoV-2 and Other Respiratory Viruses Detection Using Sialic Acid Fabricated Gold Nanoparticles.
Alfassam, Haya A; Nassar, Majed S; Almusaynid, Manal M; Khalifah, Bashayer A; Alshahrani, Abdullah S; Almughem, Fahad A; Alshehri, Abdullah A; Alawad, Majed O; Massadeh, Salam; Alaamery, Manal; Aldeailej, Ibrahim M; Alamri, Aref A; Binjomah, Abdulwahab Z; Tawfik, Essam A.
  • Alfassam HA; KACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Nassar MS; National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Almusaynid MM; National Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Khalifah BA; National Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Alshahrani AS; Microbiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi Arabia.
  • Almughem FA; National Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Alshehri AA; National Center for Pharmaceutical Technology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Alawad MO; Material Science Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Massadeh S; KACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Alaamery M; Developmental Medicine Department, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh 11481, Saudi Arabia.
  • Aldeailej IM; KACST-BWH Centre of Excellence for Biomedicine, Joint Centers of Excellence Program, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia.
  • Alamri AA; Developmental Medicine Department, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh 11481, Saudi Arabia.
  • Binjomah AZ; Microbiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi Arabia.
  • Tawfik EA; Microbiology Department, Riyadh Regional Laboratory, Ministry of Health, Riyadh 12746, Saudi Arabia.
Pharmaceutics ; 13(4)2021 Apr 06.
Article in English | MEDLINE | ID: covidwho-1178386
Semantic information from SemMedBD (by NLM)
1. Epithelial Cells PART_OF Lung
Subject
Epithelial Cells
Predicate
PART_OF
Object
Lung
2. Spectrophotometry USES Visceral manipulation
Subject
Spectrophotometry
Predicate
USES
Object
Visceral manipulation
3. Nail-Patella Syndrome AFFECTS Pale yellow
Subject
Nail-Patella Syndrome
Predicate
AFFECTS
Object
Pale yellow
4. Epithelial Cells PART_OF Lung
Subject
Epithelial Cells
Predicate
PART_OF
Object
Lung
5. Spectrophotometry USES Visceral manipulation
Subject
Spectrophotometry
Predicate
USES
Object
Visceral manipulation
6. Nail-Patella Syndrome AFFECTS Pale yellow
Subject
Nail-Patella Syndrome
Predicate
AFFECTS
Object
Pale yellow
ABSTRACT
Sialic acid that presents on the surface of lung epithelial cells is considered as one of the main binding targets for many respiratory viruses, including influenza and the current coronavirus (SARS-CoV-2) through the viral surface protein hemagglutinin. Gold nanoparticles (Au NPs) are extensively used in the diagnostic field owing to a phenomenon known as 'surface plasmonic resonance' in which the scattered light is absorbed by these NPs and can be detected via UV-Vis spectrophotometry. Consequently, sialic acid conjugated Au NPs (SA-Au NPs) were utilized for their plasmonic effect against SARS-CoV-2, influenza B virus, and Middle-East respiratory syndrome-related coronavirus (MERS) in patients' swab samples. The SA-Au NPs system was prepared by a one-pot synthesis method, through which the NPs solution color changed from pale yellow to dark red wine color, indicting its successful preparation. In addition, the SA-Au NPs had an average particle size of 30 ± 1 nm, negative zeta potential (-30 ± 0.3 mV), and a UV absorbance of 525 nm. These NPs have proven their ability to change the color of the NPs solutions and patients' swabs that contain SARS-CoV-2, influenza B, and MERS viruses, suggesting a rapid and straightforward detection tool that would reduce the spread of these viral infections and accelerate the therapeutic intervention.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Pharmaceutics13040502

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Language: English Year: 2021 Document Type: Article Affiliation country: Pharmaceutics13040502